May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Participation of Bone Marrow Derived Mesenchymal Stem Cells in Corneal Epithelial Wound Healing
Author Affiliations & Notes
  • H. Zhang
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • C. Xu
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • L. Liang
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • D. Yu
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Z. Liu
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Footnotes
    Commercial Relationships  H. Zhang, None; C. Xu, None; L. Liang, None; D. Yu, None; Z. Liu, None.
  • Footnotes
    Support  National Natural Science Foundation of China (30371514 and 30600697), the Key Technologies Research and Development Program of Guangdong Province (2003A30201)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 6071. doi:https://doi.org/
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    • Get Citation

      H. Zhang, C. Xu, L. Liang, D. Yu, Z. Liu; Participation of Bone Marrow Derived Mesenchymal Stem Cells in Corneal Epithelial Wound Healing. Invest. Ophthalmol. Vis. Sci. 2008;49(13):6071. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Bone marrow-derived mesenchymal stem cells (MSCs) have long been known to be multi-potent with the ability to differentiate into various cells of ectodermal, mesodermal, and endodermal origins. Recent reports have also indicated that transplantation of BMMSCs can reconstruct the damaged rat corneal surface. However, whether MSCs have the ability to differentiate into corneal epithelial cells, and the dynamics of these cells after transplantation remain unclear. The goal of the present study is to investigate the potential of human MSCs in reconstruction of corneal epithelium during wound healing process.

Methods: : We followed, using a corneal epithelial reconstruction assay, 5-bromo-2’-deoxy-uridine (BrdU) labeled human MSCs transplanted into rabbit corneal limbus with central epithelial debridement. Controls were created by transplanting of the same cells into rabbits without corneal wounds.

Results: : Our results indicate that human MSCs can migrate in rabbit cornea during corneal wound healing and recovered corneal epithelia containing MSCs can express specific markers of corneal epithelium (Keratin 3) as normal animals. Wounding could be a stimulating factor for the migration of MSCs on the rabbit corneal surface.

Keywords: cornea: epithelium • cornea: basic science • microscopy: light/fluorescence/immunohistochemistry 
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