May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Expression Patterns of Nucleostemin (Gnl3) in Mouse and Human Cornea
Author Affiliations & Notes
  • S. Yoshida
    Ophthalmology, School of Medicine, Keio University, Tokyo, Japan
    Cornea Center,
    Tokyo Dental College, Ichikawa, Japan
  • S. Shimmura
    Ophthalmology, School of Medicine, Keio University, Tokyo, Japan
  • H. Miyashita
    Ophthalmology, School of Medicine, Keio University, Tokyo, Japan
  • N. Kato
    Ophthalmology, School of Medicine, Keio University, Tokyo, Japan
  • J. Shimazaki
    Ophthalmology,
    Tokyo Dental College, Ichikawa, Japan
  • K. Tsubota
    Ophthalmology, School of Medicine, Keio University, Tokyo, Japan
  • Footnotes
    Commercial Relationships  S. Yoshida, None; S. Shimmura, None; H. Miyashita, None; N. Kato, None; J. Shimazaki, None; K. Tsubota, None.
  • Footnotes
    Support  JSPS Grant-in-Aid for Scientific Research (KAKENHI 18791302)
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 6073. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      S. Yoshida, S. Shimmura, H. Miyashita, N. Kato, J. Shimazaki, K. Tsubota; Expression Patterns of Nucleostemin (Gnl3) in Mouse and Human Cornea. Invest. Ophthalmol. Vis. Sci. 2008;49(13):6073. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : . Nucleostemin (Gnl3) is a candidate marker for stem cells. It is expressed in the nucleolus of various stem cells such as embryonic stem cells, neural stem cells and also in cancer stem cells, but not in most differentiated cells. Gnl3 is thought to be involved in regulation of cell cycle and proliferation. To investigate the possible use of Gnl3 as a marker for corneal stem cells, we examined the expression patterns of Gnl3 in human and mouse cornea.

Methods: : Expression of Gnl3 in normal human and mouse corneal epithelium was examined by RT-PCR and immunohistochemistry. The expression in wounded mouse corneal epithelium and in cultured human corneal epithelial cells was also examined. Co-expression of Gnl3 with the stem cell markers p63, K15 and K19, and with the proliferation marker Ki67 was also examined by immunohistochemistry.

Results: : Gnl3 expression was detected in both human and mouse corneal epithelium by RT-PCR. Immunohistochemistry revealed that Gnl3 was expressed predominantly in basal cells of the limbal area through the conjunctiva. In addition, Gnl3 was also expressed in suprabasal cells, including the central area of corneal epithelium, although the level was not as high. Furthermore, expression was found in keratocytes and endothelial cells, which are thought to be quiescent. The expression of Gnl3 was not associated with the expression of K15, K19, or Ki67 but overlapped with the expression of p63. Wound repair-associated upregulation of Gnl3 was also observed.

Keywords: cornea: epithelium • gene/expression • immunohistochemistry 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×