May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Decreased Expression of Inducible Hsp70 Under in vivo Systemic Glutathione Depletion in the Mouse Retina
Author Affiliations & Notes
  • K. Cho
    Ophthalmology and Visual Science, Catholic Medical Center, Seoul, Republic of Korea
  • M. Park
    Ophthalmology and Visual Science, Catholic Medical Center, Seoul, Republic of Korea
  • J. Moon
    Ophthalmology and Visual Science, Catholic Medical Center, seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  K. Cho, None; M. Park, None; J. Moon, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 6108. doi:https://doi.org/
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    • Get Citation

      K. Cho, M. Park, J. Moon; Decreased Expression of Inducible Hsp70 Under in vivo Systemic Glutathione Depletion in the Mouse Retina. Invest. Ophthalmol. Vis. Sci. 2008;49(13):6108. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Heat shock protein(HSP)70 has been known to have neuroprotective effect on the retina in vitro and in a rat glaucoma model. This study was to evaluate the inducible HSP70 expression after oxidative stress induced by glutathione depletion in the mouse retina.

Methods: : Unregulated oxidative stress was induced by depletion of intracellular glutathione by systematic administration of buthionine sulphoximine (BSO), an inhibitor of gamma-glutamylcysteine synthetase. After 0, 1, 4 and 7 days of BSO administration, we examined expression of HSP by real time RT-PCR, immunoblot, and immunohistochemistry assay.

Results: : From analysis of RT-PCR and immunoblot assay, expression of HSP70 was observed in control group(Day 0) and significantly decreased from 1day after BSO injection. Immunohistochemistry assay showed that expression of HSP70 was most prominent in photoreceptor layer among retinal layers and that depletion of glutathione decreased HSP70’s expression to a great extent in the ganglion cell layer.

Conclusions: : HSP seems to be play important role in prevention of oxidative stress induced retinal cell injury. But, sudden depletion of glutathione may decrease the expression of HSP70 by down regulation. And ganglion cell layer is more vulnerable to oxidative stress than the other layers in retina.

Keywords: oxidation/oxidative or free radical damage • retina • ganglion cells 
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