May 2008
Volume 49, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2008
Endoplasmic Reticulum Stress Is Associated With Retinal Neuronal Cell Death Induced by Ischemia-Reperfusion Injury
Author Affiliations & Notes
  • N. Hata
    Ophthalmology, Chibe University Sch of Medicine, Chiba, Japan
  • T. Oshitari
    Ophthalmology, Chibe University Sch of Medicine, Chiba, Japan
  • A. Yokoyama
    Ophthalmology, Chibe University Sch of Medicine, Chiba, Japan
  • Y. Mitamura
    Ophthalmology, Chibe University Sch of Medicine, Chiba, Japan
  • S. Yamamoto
    Ophthalmology, Chibe University Sch of Medicine, Chiba, Japan
  • Footnotes
    Commercial Relationships  N. Hata, None; T. Oshitari, None; A. Yokoyama, None; Y. Mitamura, None; S. Yamamoto, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2008, Vol.49, 6119. doi:https://doi.org/
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      N. Hata, T. Oshitari, A. Yokoyama, Y. Mitamura, S. Yamamoto; Endoplasmic Reticulum Stress Is Associated With Retinal Neuronal Cell Death Induced by Ischemia-Reperfusion Injury. Invest. Ophthalmol. Vis. Sci. 2008;49(13):6119. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To determine whether the ER stress-mediated apoptosis signal-regulating kinase 1 (ASK1)-c-Jun N-terminal kinase (JNK) pathway is associated with the degeneration of the retinal neurons induced by ischemia-reperfusion injury.

Methods: : The rat retinal ischemia-reperfusion model was used. After 60 minutes of ischemia, the retinas were isolated and fixed after 6, 9, 12, 18, 24 hours, 2, 5 and 9 days of reperfusion. Cryosections were immunostained with Fluoro-Jade B, a degenerating neuron marker. Semi-quantitative analysis of the expression of IRE1α, ASK1, SAPK/ERK kinase 1 (SEK1), and JNK were performed in both control and ischemic retinas.

Results: : In ischemic retinas, the intensities of IRE1α immunoreactivity in the ganglion cell layer (GCL) were significantly increased compared to those in the control retinas. In ischemic retinas, the numbers of SEK1-, ASK1-, and JNK-positive cells were significantly higher in the GCL compared to those in the control retinas. The cells that were positive for SEK1-, ASK1-, and JNK were also positive for Fluoro-Jade B-positive cells.

Conclusions: : These results indicate that the ASK1-JNK pathway induced by ER stress was associated with the neuronal cell death after ischemia-reperfusion injury in rat retinas.

Keywords: retina • stress response • retinal degenerations: cell biology 
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