May 2007
Volume 48, Issue 13
ARVO Annual Meeting Abstract  |   May 2007
Assesment of Laser Tissues Interaction Using Fundus Autofluorescence
Author Affiliations & Notes
  • E. F. van Kuijk
    Univ of Texas Medical Branch, Galveston, Texas
    Ophthalmology & Visual Sciences,
  • B. Bell
    Univ of Texas Medical Branch, Galveston, Texas
    Center for Biomedical Engineering,
  • R. Johnston
    Univ of Texas Medical Branch, Galveston, Texas
    Center for Biomedical Engineering,
  • M. Motamedi
    Univ of Texas Medical Branch, Galveston, Texas
    Center for Biomedical Engineering,
  • Footnotes
    Commercial Relationships E.F. van Kuijk, None; B. Bell, None; R. Johnston, None; M. Motamedi, None.
  • Footnotes
    Support AFOSR grant # FA-9550-06-10082 and Wilkins AMD fund.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 141. doi:
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      E. F. van Kuijk, B. Bell, R. Johnston, M. Motamedi; Assesment of Laser Tissues Interaction Using Fundus Autofluorescence. Invest. Ophthalmol. Vis. Sci. 2007;48(13):141. doi:

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose:: Fundus autofluorescence is now more widely being used for phenotyping of inherited retinal disease. Fluorescence imaging may provide a highly sensitive method for non-invasive quantitative assessment of laser-induced lesions in the retina by monitoring of changes in autofluorescence of fluorophores in the retinal pigment epithelium (RPE) following laser function of exposure parameters

Methods:: Retired female breeding stock Brown-Norway rats were purchased at an approximate age of 6 months. A modified Heidelberg Retina Angiograph (HRA I) was used to obtain images of the fundus by either infra red (IR) reflectance or autofluorescence using excitation wavelengths of 488 and 514 nm. A LaserTek Model 41 AK laser operating from 488-514nm in conjunction with a Zeiss Model 30 SL-M slit lamp was used to deliver laser radiation and induce lesions. Various powers ranging from 10-40mW were used to obtain both suprathreshold and subthreshold lesions. Subthreshold is defined a laser application that does not cause any minimal visible lesion (MVL). The lesions were imaged immediately post-irradiation and weekly for 5 weeks to follow any changes.

Results:: Individual lesions, both suprathreshold and subthreshold, were easily visualized immediately following laser treatment using HRA I in 488 and 514 autofluoresence mode. In fluorescence mode laser lesions appear as spots with reduced fluorescence relative to a broadly fluorescent background. In contrast, the same lesions visible by fluorescence were difficult to visualize in IR reflectance mode. Animals followed on a weekly basis reveal dynamic changes that begin with decreased autofluorescence immediately after laser treatment to increased autofluorescence at five weeks follow up.

Conclusions:: Autufluorescence imaging of retina shows that alterations to the RPE occur at laser powers below those that cause minimal visible lesions and offer potentially a highly sensitive technique for assessing the influence of exposure parameters on laser-induced lesion in the retina.

Keywords: imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • laser • retinal pigment epithelium 

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