May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
B7-H3 Is Necessary for Corneal Allograft Survival
Author Affiliations & Notes
  • H. Taniguchi, IV
    Ophthalmology, Nippon Medical School, Bunkyo-ku, Japan
  • J. Hori
    Ophthalmology, Nippon Medical School, Bunkyo-ku, Japan
  • M. C. Wang
    Ophthalmology, Nippon Medical School, Bunkyo-ku, Japan
  • Y. Kitahara
    Ophthalmology, Nippon Medical School, Bunkyo-ku, Japan
  • H. Takahashi
    Ophthalmology, Nippon Medical School, Bunkyo-ku, Japan
  • M. Oshima
    Immunology, National Institute of Infectious Disease, Tokyo, Japan
  • H. Yagita
    Immunology, Juntendo University School of Medicine, Tokyo, Japan
  • Footnotes
    Commercial Relationships H. Taniguchi, None; J. Hori, None; M.C. Wang, None; Y. Kitahara, None; H. Takahashi, None; M. Oshima, None; H. Yagita, None.
  • Footnotes
    Support Grant-in-Aid for Scientific Research(C) from the Japan Society for the Promotion of Science
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 191. doi:https://doi.org/
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    • Get Citation

      H. Taniguchi, IV, J. Hori, M. C. Wang, Y. Kitahara, H. Takahashi, M. Oshima, H. Yagita; B7-H3 Is Necessary for Corneal Allograft Survival. Invest. Ophthalmol. Vis. Sci. 2007;48(13):191. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: We have previously reported that B7-H1 (PD-L1), a new member of the B7 family is constitutively expressed on corneal endothelium and maintains ocular immunosuppressive microenvironments to promote survival of corneal allografts. B7-H3 has been recently identified as another new member of the B7 family, but its expression and function in the eye remain largely unknown. To determine the role of B7-H3 in ocular immune privilege, we used in vivo experimental models including anterior chamber-associated immune deviation (ACAID) and acceptance of corneal allografts.

Methods:: Normal corneas of C57BL/6 were transplanted orthotopically into normal eyes of BALB/c mice, and graft survival was assessed clinically. In a separate experiment, BALB/c mice received anterior chamber injection of C57BL/6 splenocytes 2 weeks prior to subcutaneous immunization. Induction of allo-specific ACAID was assessed by ear challenge with C57BL/6 splenocytes at 1 week after immunization. Recipients were administrated intraperitoneally with 0.2 mg of anti-B7-H3 monoclonal antibody or control rat IgG, three times a week for 8 weeks after corneal grafting, and for 3 weeks after AC injection. Expression of B7-H3 in normal and in graft-bearing eyes was assessed immunohistochemically by confocal microscopy.

Results:: B7-H3 was expressed on the corneal endothelium and iris-ciliary body of both normal corneas and allografts. Treatment with anti-B7-H3 monoclonal antibody abolished allo-specific ACAID induction. Allograft-survival in the recipients treated with anti-B7-H3 monoclonal antibody were significantly less than that in the control recipients at 8 weeks.

Conclusions:: B7-H3 is constitutively expressed on corneal endothelium and iris-ciliary body. B7-H3 plays important role in the maintenance of ocular immune privilege, such as ACAID and acceptance of corneal allografts.

Keywords: immune tolerance/privilege • transplantation • ACAID 
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