May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
The Natural History of Retinal Ganglion Cell Dysfunction and Its Relationship With IOP in DBA2/J Mice
Author Affiliations & Notes
  • M. Saleh
    Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
  • M. Nagaraju
    Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
  • V. Porciatti
    Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida
  • Footnotes
    Commercial Relationships M. Saleh, Allergan Inc, F; M. Nagaraju, None; V. Porciatti, None.
  • Footnotes
    Support NIH RO3 EY016322, NIH center grant P30-EY14801, unrestricted grant to the University of Miami from Research to Prevent Blindness, Inc. Allergan Inc supporting Maher Saleh
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 210. doi:https://doi.org/
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    • Get Citation

      M. Saleh, M. Nagaraju, V. Porciatti; The Natural History of Retinal Ganglion Cell Dysfunction and Its Relationship With IOP in DBA2/J Mice. Invest. Ophthalmol. Vis. Sci. 2007;48(13):210. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: To characterize the natural history of retinal ganglion cell (RGC) dysfunction, as measured by pattern electroretinogram (PERG) and its relationship with IOP in glaucomatous DBA/2J mice.

Methods:: IOP and PERG were longitudinally recorded (one month steps) in anesthetized (Ketamine/ Xylazine) DBA 2/J mice (n=32, 64 eyes). IOP was measured with a rebound tonometer (Tonolab, Colonial Medical Supply). PERG was recorded in response to high contrast (95%) alternating (1 Hz) gratings (0.05 cycles/deg, 50 x 56 deg field size). Diffuse light flashes on an adapting background (FERG) were also recorded as an index of outer retina activity. After the follow up period, eyes were histologically processed to evaluate the thickness of the retinal nerve fiber layer (RNFL).

Results:: IOP increased moderately between 2 and 6 months with a progression of 0.92 mm Hg/month. After 6 months the IOP displayed a steeper increase and tended to level off by 11 months. The PERG amplitude decreased progressively after 2 months of age to reach the noise level (85% amplitude loss) at about 10-11 months, at which age histological analysis showed a relatively minor loss of retinal nerve fiber layer (RNFL) thickness (-39±18%). IOP and PERG changes with time were highly correlated (p<0.001). Between 2 and 11 months the cone-flash ERG did not show significant changes.

Conclusions:: In DBA/2J mice inner retina function progressively decreases after 2 months of age and it is virtually abolished by 10-11 months, while outer retina function is unchanged. In keeping with previous optic nerve histology (Libby et al, Vis Neurosci 2005), at 10-11 months the RNFL thickness is relatively spared, thereby indicating that RGC dysfunction may precede RGC death. Progression of inner retina dysfunction is strongly associated with progressive IOP increase.

Keywords: ganglion cells • degenerations/dystrophies • electrophysiology: non-clinical 
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