May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
ßB2-Crystallin Expressed in Retinal Ganglion Cells After NMDA-Induced Retinal Damage
Author Affiliations & Notes
  • N. Senda
    Ophthalmology, Shinshu Univ Schl of Medicine, Matsumoto, Japan
  • T. Kyoumoto
    Ophthalmology, Shinshu Univ Schl of Medicine, Matsumoto, Japan
  • T. Yanagidaira
    Ophthalmology, Shinshu Univ Schl of Medicine, Matsumoto, Japan
  • H. Imai
    Ophthalmology, Shinshu Univ Schl of Medicine, Matsumoto, Japan
  • N. Katai
    Ophthalmology, Shinshu Univ Schl of Medicine, Matsumoto, Japan
  • Footnotes
    Commercial Relationships N. Senda, None; T. Kyoumoto, None; T. Yanagidaira, None; H. Imai, None; N. Katai, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 233. doi:
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    • Get Citation

      N. Senda, T. Kyoumoto, T. Yanagidaira, H. Imai, N. Katai; ßB2-Crystallin Expressed in Retinal Ganglion Cells After NMDA-Induced Retinal Damage. Invest. Ophthalmol. Vis. Sci. 2007;48(13):233.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: We previously screened the gene expression profile in retinal ischemia reperfusion injury by using the DNA microarray system and discovered many kinds of ß and γ-Crystallin gene were expressed in injured rat retina. However, the function of ß and γ-Crystallin expressed in extralenticular tissues is unknown. Our purpose is to characterize the ßB2-Crystallin induced in retinal ganglion cells after NMDA-receptor-mediated retinal apoptosis.

Methods:: Intravitreal injections of 4nmol of NMDA were performed to male C57BL/6J mice (20-30g) after anesthesia. Retinas were collected at various time points after NMDA injection, and both protein and gene expression levels were measured by using Western blotting and real-time RT-PCR methods, respectively. Localization of ßB2-Crystallin proteins in injured retina was determined using the immunohistochemical methods.

Results:: On Western blotting, ßB2-Crystallin was induced in the retina after intravitreal injection of NMDA. The peak time point was at 6 hours after NMDA injection. ßB2-Crystallin gene expression level was gradually increased after NMDA injection. Immunohistochemical study showed that ßB2-Crystallin was expressed mainly in retinal ganglion cells but not in apoptosis cells.

Conclusions:: ßB2-Crystallin was induced in the retinal ganglion cells after intravitreal injection of NMDA.

Keywords: crystallins • ganglion cells • apoptosis/cell death 
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