Abstract
Purpose::
We presented that ßB2-crystallin protein and mRNA were upregulated in rat retinal ischemia-reperfusion injury and the peak time point of these expression was at 12 hours after reperfusion in ARVO, 2006. To investigate the roles ßB2-crystallin played in rat retinal ischemia-reperfusion injury by using the ßB2-crystallin short interfering RNA (siRNA).
Methods::
Retinal ischemia for 60 minutes was induced in rats by increasing the intraocular pressure to 110 mm Hg. To inhibit the upregulation of ßB2-crystallin, intravitreal injection of ßB2-crystallin siRNA was performed before ischemia. The expression of ßB2-crystallin was studied on Western blotting and immunohistochemical methods.
Results::
On Western blotting, ßB2-crystallin was downregulated at 12 hours after reperfusion. On immunohistochemical methods, the number of cells in the ganglion cell layer changed at 24 hours after ischemia-reperfusion injury.
Conclusions::
ßB2-crystallin may regulate the retinal ganglion cell death after ischemia-reperfusion injury.
Keywords: crystallins • ganglion cells • apoptosis/cell death