May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Light-Induced Changes in the Expression of Several Calcium-Binding Proteins in the Retina of Pigmented Rabbits
Author Affiliations & Notes
  • M. J. Perez Carrasco
    Optica II, Escuela Univ de Optica, Madrid, Spain
  • J. Vega
    Anatomía, Facultad de Medicina, San Pablo CEU, Madrid, Spain
  • C. Sanchez-Ramos
    Optica II, Escuela Univ de Optica, Madrid, Spain
  • A. Germana
    Faculta di Medicia Veterinaria, Universita de Messina, Madrid, Spain
  • Mª. Moral-Martinez
    Optica II, Escuela Univ de Optica, Madrid, Spain
  • M. Elias de Tejada-Casanova
    Oftalmología, Hospital Clínico San Carlos, Madrid, Spain
  • F. Daza
    Optica II, Escuela Univ de Optica, Madrid, Spain
  • Mª. Casas-Builla
    Optica II, Escuela Univ de Optica, Madrid, Spain
  • J. Benitez del Castillo
    Oftalmología, Facultad de Medicina, UCM, Madrid, Spain
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 32. doi:
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      M. J. Perez Carrasco, J. Vega, C. Sanchez-Ramos, A. Germana, Mª. Moral-Martinez, M. Elias de Tejada-Casanova, F. Daza, Mª. Casas-Builla, J. Benitez del Castillo; Light-Induced Changes in the Expression of Several Calcium-Binding Proteins in the Retina of Pigmented Rabbits. Invest. Ophthalmol. Vis. Sci. 2007;48(13):32.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: Calcium ion (Ca2+) plays several key roles for the physiology of cells, including photoreceptors and neurons in the vertebrate retina. Among the mechanisms involved in the maintenance of intracellular Ca2+ homeostasis, there are a series of proteins collectively known as Ca2+-binding proteins. Changes in the expression of some of these proteins are at the basis of some degenerative diseases.To check whether this also occurs in retinopathy associated to light exposure or not, we have analyzed immunohistochemically the occurrence of calretinin, parvalbumin, calbindin-D 28k, neurocalcin and S100 protein in the retina of pigmented rabbits exposed to circadian light.

Methods:: In order to analyze the type of light responsible for the retinal light-induced changes, we implanted intraocular lens to filter the blue segments of the visual spectrum. All the investigated Ca2+-binding proteins were found in the retina of control and experimental animals with a heterogeous pattern of expression in neurons and non-neuronal cells, especially photoreceptors.

Results:: Light exposure resulted in a decrease in the expression of calretinin, parvalbumin and calbindin-D 28 k, but not in variations in the expression of neurocalcin or S100 protein. Interestingly, intraocular filters for the blue component of the light spectrum partially improved the decreased expression of those proteins.

Conclusions:: As a whole, the present results suggest that an alteration in the Ca2+ homeostatic mechanism, involving Ca2+-binding proteins, might account for cell light-induced retinal pathologies.

Keywords: age-related macular degeneration • calcium • protein structure/function 
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