May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Fundus Autofluorescence in a Rabbit Model of Age-Related Macular Degeneration Induced by Advanced Glycation End Product Microspheres as Imitation Lipofuscin
M. Hirata; T. Yasukawa; P. Wiedemann; N. Kuno; E. Kimura; A. Takase; S. Hoffmann; W. Eichler; Y. Ogura
Author Affiliations & Notes
  • M. Hirata
    Ophthalmology and visual science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
  • T. Yasukawa
    Ophthalmology and visual science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
    Department of Ophthalmology, University of Leipzig, Leipzig, Germany
  • P. Wiedemann
    Department of Ophthalmology, University of Leipzig, Leipzig, Germany
  • N. Kuno
    Ophthalmology and visual science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
    Research and Development Center, Santen Pharmaceuticals Co., Ltd, Ikoma, Japan
  • E. Kimura
    Research and Development Center, Santen Pharmaceuticals Co., Ltd, Ikoma, Japan
  • A. Takase
    Ophthalmology and visual science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
  • S. Hoffmann
    Department of Ophthalmology, University of Leipzig, Leipzig, Germany
  • W. Eichler
    Department of Ophthalmology, University of Leipzig, Leipzig, Germany
  • Y. Ogura
    Ophthalmology and visual science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
  • Footnotes
    Commercial Relationships M. Hirata, None; T. Yasukawa, None; P. Wiedemann, None; N. Kuno, None; E. Kimura, None; A. Takase, None; S. Hoffmann, None; W. Eichler, None; Y. Ogura, None.
  • Footnotes
    Support Grant-in-Aid for Scientific Research No. 18591929 from Japan Society for the Promotion of Science
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 41. doi:
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      M. Hirata, T. Yasukawa, P. Wiedemann, N. Kuno, E. Kimura, A. Takase, S. Hoffmann, W. Eichler, Y. Ogura; Fundus Autofluorescence in a Rabbit Model of Age-Related Macular Degeneration Induced by Advanced Glycation End Product Microspheres as Imitation Lipofuscin. Invest. Ophthalmol. Vis. Sci. 2007;48(13):41.

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Abstract

Purpose:: Recent researches suggested that fundus autofluorescence (FAF) was mainly derived from lipofuscin accumulation and possibly associated with the pathogenesis of age-related macular degeneration (AMD). The object of this study was to observe the distribution and the pattern of FAF in our rabbit model of AMD induced by subretinal implantation of glycoxidized albumin-microspheres as imitation lipofuscin.

Methods:: Glycoxidized microspheres were prepared with four-day-incubation of water-in-oil emulsions of serum albumin plus glycolaldehyde to allow glycoxidation and consequent crosslinking. In anesthetized rabbits, 250 µg of this imitation lipofuscin was injected into the subretinal space. FAF was observed at weeks 1, 4, 8 and 12 by Optical Coherence Tomography (OCT) and Heidelberg Retinal Angiography (HRA). The eyes enucleated at week 4 or 12 were evaluated by light and fluorescein microscopy.

Results:: OCT revealed that the retina in the area with implantation of imitation lipofuscin had been reattached at week 1. HRA showed FAF related to implanted imitation lipofuscin. Different patterns of FAF were exhibited, similar to pathological FAF patterns in human. At weeks 4 and 12, fundoscopical and histological examination disclosed accumulation of imitation lipofuscin in retinal pigment epithelial cells and drusen formation in all cases and type 1 choroidal neovascularization in some cases.

Conclusions:: Our rabbit model of AMD could simulate FAF patterns observed in human aged eyes with or without AMD. This model may be useful to elucidate the relationship of FAF, lipofuscin, and AMD and to histologically investigate the lesion with various patterns of FAF.

Keywords: age-related macular degeneration • drusen • imaging/image analysis: non-clinical 
© 2007, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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