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M. Hirata, T. Yasukawa, P. Wiedemann, N. Kuno, E. Kimura, A. Takase, S. Hoffmann, W. Eichler, Y. Ogura; Fundus Autofluorescence in a Rabbit Model of Age-Related Macular Degeneration Induced by Advanced Glycation End Product Microspheres as Imitation Lipofuscin. Invest. Ophthalmol. Vis. Sci. 2007;48(13):41.
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Recent researches suggested that fundus autofluorescence (FAF) was mainly derived from lipofuscin accumulation and possibly associated with the pathogenesis of age-related macular degeneration (AMD). The object of this study was to observe the distribution and the pattern of FAF in our rabbit model of AMD induced by subretinal implantation of glycoxidized albumin-microspheres as imitation lipofuscin.
Glycoxidized microspheres were prepared with four-day-incubation of water-in-oil emulsions of serum albumin plus glycolaldehyde to allow glycoxidation and consequent crosslinking. In anesthetized rabbits, 250 µg of this imitation lipofuscin was injected into the subretinal space. FAF was observed at weeks 1, 4, 8 and 12 by Optical Coherence Tomography (OCT) and Heidelberg Retinal Angiography (HRA). The eyes enucleated at week 4 or 12 were evaluated by light and fluorescein microscopy.
OCT revealed that the retina in the area with implantation of imitation lipofuscin had been reattached at week 1. HRA showed FAF related to implanted imitation lipofuscin. Different patterns of FAF were exhibited, similar to pathological FAF patterns in human. At weeks 4 and 12, fundoscopical and histological examination disclosed accumulation of imitation lipofuscin in retinal pigment epithelial cells and drusen formation in all cases and type 1 choroidal neovascularization in some cases.
Our rabbit model of AMD could simulate FAF patterns observed in human aged eyes with or without AMD. This model may be useful to elucidate the relationship of FAF, lipofuscin, and AMD and to histologically investigate the lesion with various patterns of FAF.
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