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E. M. Rocha, C. M. Modulo, A. G. Jorge, A. M. Braz, R. C. Lataro, A. A. Jordão, Jr., R. Bertazolli Filho, J. S. Paula, A. C. Dias; Thyroid Hormones Influence Lacrimal Gland and Ocular Surface and Modulate Thyroid Hormone Receptor ß-1. Invest. Ophthalmol. Vis. Sci. 2007;48(13):416.
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Hormone diseases induce changes in the lacrimal glands (LG) and ocular surface (OS). Thyroid hormone (TH) induces cell proliferation and lipid metabolism through activation of thyroid hormone receptors. The aim of the present study was to evaluate the location and comparative expression of thyroid hormone receptor ß-1 (TRß-1) in LG of rats with hypothyroidism and controls and the impact of this disease on LG and OS structure and function.
Hypothyroidism was induced in Wistar male rats by the chronic use of tiamazole. Ten weeks later corneal cells were collected for impression cytology (IC). Rats were euthanized and tissues evaluated by immunoperoxidase staining and western blot for TRß-1. The content of malondialdehyde (MDA) and acetylcholine (Ach) in LG was determined by spectrophotometry (n=5/group in all experiments).
LG weight was significantly lower in hypothyroid rats (P<0.05). Western blot indicated that LG express TRß1 and that hypothyroidism induces a higher expression of this receptor. IC was significantly different and Ach was significantly lower in hypothyroid rats (P<0.05).
Chronically reduced levels of TH lead to biochemical and structural changes and modulate the levels of TRß1 in LG. These events confirm that LG is a target organ for TH and may help to understand the mechanism related to dry eye in hypothyroidism.
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