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S. Ishikawa, J. Nakabayashi, N. Nakabayashi, A. Hirata, S. Okinami; Neuroprotective Effect of Small Interfering RNA Targeted Against Caspase 3 on Rat Retinal Ganglion Cells Induced by Ischemia Reperfudion Injury in Rat. Invest. Ophthalmol. Vis. Sci. 2007;48(13):564.
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To investigate the neuroprotective effects of caspase-3 siRNA against ischemia-reperfusion injury in rat eyes.
Experiments were performed in Wister rat (male, 7 weeks of age). The retinal ischemia was induced by increase of the intraocular pressure to 130mmHg for 45min. SiRNA against rat caspase-3 (170pmol / 5ul) or PBS (5ul) for control was injected into vitreous space prior to induction of retinal ischemia. Three days later, each eye was enucleated and analyzed by counting the number of retinal ganglion cells (RGCs). Differences were analyzed by paired t-test.
In control eyes, the number of RGCs were reduced significantly as against normal eyes (RGCs: 463.0±140.0 vs 697.1±194.4 cells/mm p=0.19).In eyes pretreated with siRNA (34mM), these were retained significantly as compared with the control eyes (RGCs: 510.0±332.4 vs 463.0±139.7 cells/mm p=0.87).
Caspase-3 siRNA may be useful in preventing retinal cell death by ischemic injury in rat eyes.
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