May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Reactive Protection of Retinal Function Related to Transplantation
Author Affiliations & Notes
  • S. Mohand Said, Sr.
    Cell and Molecule Physiopathology of Retina, Inserm U592, Hopital Saint Antoine, Université de Paris VI, Paris Cedex 12, France
    Ophthalmology Department, Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Paris, France
  • Y. Yang
    Cell and Molecule Physiopathology of Retina, Inserm U592, Hopital Saint Antoine, Université de Paris VI, Paris Cedex 12, France
  • M. Simonutti
    Cell and Molecule Physiopathology of Retina, Inserm U592, Hopital Saint Antoine, Université de Paris VI, Paris Cedex 12, France
  • V. Fontaine
    Cell and Molecule Physiopathology of Retina, Inserm U592, Hopital Saint Antoine, Université de Paris VI, Paris Cedex 12, France
  • T. Léveillard
    Cell and Molecule Physiopathology of Retina, Inserm U592, Hopital Saint Antoine, Université de Paris VI, Paris Cedex 12, France
  • J. A. Sahel
    Cell and Molecule Physiopathology of Retina, Inserm U592, Hopital Saint Antoine, Université de Paris VI, Paris Cedex 12, France
    Ophthalmology Department, Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Paris, France
  • Footnotes
    Commercial Relationships S. Mohand Said, None; Y. Yang, None; M. Simonutti, None; V. Fontaine, None; T. Léveillard, None; J.A. Sahel, None.
  • Footnotes
    Support Fédération des Aveugles et Handicapés Visuels de France
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 622. doi:
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      S. Mohand Said, Sr., Y. Yang, M. Simonutti, V. Fontaine, T. Léveillard, J. A. Sahel; Reactive Protection of Retinal Function Related to Transplantation. Invest. Ophthalmol. Vis. Sci. 2007;48(13):622.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: Our aim is to demonstrate that transplantation of neural retina or selected photoreceptor not only limits death of cones, but also prevents loss of visual function by using a retinal degeneration model-P23H rat.

Methods:: Heterozygote P23H rats (3months) were transplanted subretinally in one eye with retinal sheets (2mm x 4mm) obtained from postnatal 8 day Sprague Drawley rats. After 6 months, transplanted retinas were analysed by immunocytochemistry and histology. We quantified the numbers of retinal cones, using a stereological approach to obtain unbiased samples after immunolabeling with peanut agglutinin (PNA) lectin to label cones and anti-opsin Rho-4D2 antibody to label rods. ERG recording was assessed for the analysis of visual function.

Results:: Histology and ERG show photoreceptor degeneration of P23H rats following stages, specially rapid from 2 to 4 months. During this period, scotopic B wave amplitude decreases faster than photopic B wave amplitude. By transplantation with neural retina (14 rats) or photoreceptor (18 rats), remaining cones were average of 9.2% (P<0.01) and 9.9% (P<0.01) more than that of the control. Both types of transplantation had dramatic effects on photopic ERG (B wave amplitude, P<0.05). Increase of cone number corresponds to rising of photopic ERG B wave amplitude.

Conclusions:: Our study provides a better understanding of photoreceptor survival and degeneration pathways, both of cell quantity and cell function. Furthermore, this fact confirms effective application of retinal sheet or photorecptor transplantation in Retinitis Pigmentosa.

Keywords: transplantation • photoreceptors • electroretinography: non-clinical 
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