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J. Zhang, Y.-Q. Lin, A. Shafiee, T. T. Lam; ICAM-1 Expression in Primary Human Retinal Endothelial Cells Exposed to Lipopolysaccharide (lps) and Cobalt Chloride (cocl2). Invest. Ophthalmol. Vis. Sci. 2007;48(13):87.
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This study was designed to evaluate the expression of intercellular adhesion molecule-1 (ICAM-1) in primary human retinal endothelial cells exposed to lipopolysaccharide (LPS) and chemically induced hypoxic responses by cobalt chloride (CoCl2).
Human retinal endothelial cells (HREC) were exposed to 1 µg/mL of LPS or 100 µM CoCl2 with or without 1 or 10 µM of SN50, a cell-permeable peptide inhibitor of nuclear factor kappa B (NFΚB), for 18h. Protein levels of ICAM-1 in the cell lysate were determined by ELISA or Western blotting.
LPS but not CoCl2 significantly increased the expression of ICAM-1. SN50 inhibited LPS-stimulated ICAM-1 expression in a dose-dependent manner.
Our data suggest that chemically induced hypoxic responses do not appear to involve ICAM-1 expression in retinal endothelial cells while LPS-stimulated ICAM-1 expression is mediated by the transcriptional factor NFΚB.
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