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H. Hoshi, I.-B. Kim, S. L. Mills; The Incidence of Ganglion Cell Coupling in the Rabbit Retina. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1161. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
Vaney (Neurosci Lett. 125:187, 1991) first demonstrated via Neurobiotin injection that some ganglion cells were coupled to their neighbors and/or amacrine cells, presumptively via gap junctions. We have extensively examined the morphology and tracer coupling patterns of ganglion cells in the rabbit retina.
We target ganglion cells with the vital fluorescent dyes acridine orange or Popro-1. We then iontophorese Neurobiotin into ganglion cells located either in the GCL and INL. Staining is visualized with streptavidin-Cy3 and imaged on a Zeiss LSM510 confocal microscope.
We have recovered examples of most of the rabbit ganglion cell catalog described by Rockhill et al. (J. Neurosci. 22:3831, 2002). Regularly coupled ganglion cells include the G1, G3, G6, G9, G10, and G11(OFF), which are all coupled to amacrine cells, and the G7, which can be coupled to neighboring ganglion cells of the same type. Displaced ganglion cells are generally medium-field in size and often coupled to amacrine cells. The ON and OFF G4 and the ON G11 ganglion cells were never tracer coupled. We also believe the G2, G5 and G8 to be uncoupled, but lower encounter rates hinder a firm conclusion.
The extent to which ganglion cells are coupled via gap junctions to amacrine cells may not be generally appreciated and suggests that this type of heterologous coupling may be an important processing mechanism in several types of visual channel. The results are generally consistent with the widespread metabolic coupling found in ganglion cells of the rabbit retina (Marc and Jones, J. Neurosci. 22:413, 2002) and with the tracer coupling patterns reported from Neurobiotin injections of ganglion cells in the rabbit retina by Xin and Bloomfield (J. Comp. Neurol. 383:512, 1997).
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