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O. P. Kocaoglu, S. R. Uhlhorn, M. Celdran, E. Hernandez, V. Porciatti, J.-M. Parel, F. Manns; Comparison of C57Bl/6 Mouse Retinal Thickness Measured Using Optical Coherence Tomography and Histology. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1198.
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© ARVO (1962-2015); The Authors (2016-present)
To compare C57Bl/6 mouse retinal thickness measured on histological sections and in vivo non-invasively using Optical Coherence Tomography (OCT).
The retinas of 5 anesthetized C57Bl/6 mice were imaged with a custom-made non-contact slit-lamp based OCT system designed for the mouse eye. Cross-sectional images of the retina were acquired at 3 weeks and at 4 weeks of age along lines located 2 to 3 optic disc diameters away from the optic disc. The position of the scan was controlled using a simultaneous fundus image. Retinal thickness was measured from the retinal pigment epithelium-photoreceptor layer interface to the vitreo-retinal interface on randomly selected A-lines within the region of interest. After the second imaging session, the mice were euthanized and the eyes were enucleated, fixed in 10% formalin, embedded in paraffin and cut into 8 microns thick sections. Histological retinal thickness was measured on sections corresponding to the location of the OCT scan. Retinal thickness measured using histology and OCT was compared.
The average total retinal thickness measured from the OCT images was 178±4 microns. The average retinal thickness measured from the histological sections was 213±17 microns. The OCT thickness was found to be within the range of previously published values obtained by OCT imaging or frozen sections (Horio et al 2001, Schmucker and Schaeffel 2004,).
The thickness obtained from histological sections was found to be significantly (19% on average) larger and more variable than the thickness obtained from OCT scans. This difference may stem from the difficulty in controlling the obliquity of the histological sections.Support: This work was supported in part by the generosity of the Wollowick Foundation; NIH grant R03 EY016322; NIH center grant P30-EY014801; Research to Prevent Blindness; The Florida Lions Eye Bank; The Henri and Flore Lesieur Foundation, West Palm Beach, FL.
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