May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Elevated TGF-ß1 Signaling in Transgenic Mice Inhibits Development of the Choroidal Vasculature
Author Affiliations & Notes
  • A. Ohlmann
    Institute of Human Anatomy and Embryology, University of Regensburg, Regensburg, Germany
  • M. Scholz
    Department of Anatomy, University of Erlangen-Nürnberg, Erlangen, Germany
  • E. R. Tamm
    Institute of Human Anatomy and Embryology, University of Regensburg, Regensburg, Germany
  • Footnotes
    Commercial Relationships A. Ohlmann, None; M. Scholz, None; E.R. Tamm, None.
  • Footnotes
    Support Supported by DFG grant Ta 115/15-1
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 1225. doi:https://doi.org/
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      A. Ohlmann, M. Scholz, E. R. Tamm; Elevated TGF-ß1 Signaling in Transgenic Mice Inhibits Development of the Choroidal Vasculature. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1225. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: To study the influence of TGF-ß1 signaling on the development of the choroidal vasculature.

Methods:: Transgenic mice with lens expression of active TGF-ß1 under control of the strong ßB1-crystallin promoter (Flügel-Koch et al., Dev. Dyn. 2002) were bred in a FVB/N or mixed FVB/N x CD31 background. The retinal and choroidal phenotypes of heterozygous ßB1-TGFß1 mice were investigated from postnatal day (P) 0 to P21 by light and electron microscopy, by staining with biotinylated Griffonia simplicifolia lectin, immunostaining for GFAP, α-smooth muscle actin, Thy-1, p-Smad2/3 and activated caspase-3, and by TUNEL labeling.

Results:: The enhanced expression of TGF-ß1 during eye development results in inhibition of choridal vascular growth as ßB1-TGFß1 mice do not develop a layer of choriocapillares underlying the outer retina. In addition, no capillaries develop in the plexiform layers of the retina. When compared to wild-type littermates, retinal neurons of ßB1-TGFß1 mice show a significant increase of apoptotic cell between P7 and P9. A second peak of apoptotic cell death is observed around P14, when apoptosis is mainly found in the outer nuclear layer. Comparable findings were seen when mice with FVB/N were compared with those with mixed FVB/N x CD31 background, in which wild-type littermates develop a normal retinal phenotype.

Conclusions:: In transgenic in vivo conditions, TGF-ß1 signaling inhibits growth of retinal and choroidal capillaries. The lack of capillaries leads to an increase in apoptotic cell death of retinal neurons.

Keywords: choroid • retina • development 
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