May 2007
Volume 48, Issue 13
ARVO Annual Meeting Abstract  |   May 2007
Evaluation of Adherence of S. epidermidis on Different IOLs
Author Affiliations & Notes
  • J. L. Alio
    Refractive Surgery, Inst Oftalmologico Alicante, Alicante, Spain
  • C. Ferrer
    Research and development, Inst Oftalmologico Alicante, Vissum, Instiuto Oftalmologico, Alicante, Spain
  • J. L. Rodriguez-Prats
    Refractive Surgery, Inst Oftalmologico Alicante, Vissum, Instituto Oftalmologico, Alicante, Spain
  • Footnotes
    Commercial Relationships J.L. Alio, None; C. Ferrer, None; J.L. Rodriguez-Prats, None.
  • Footnotes
    Support FIS Grant CO3/13 "Investigacion clinica y basica para la prevencion de la ceguera"
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 740. doi:
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    • Get Citation

      J. L. Alio, C. Ferrer, J. L. Rodriguez-Prats; Evaluation of Adherence of S. epidermidis on Different IOLs. Invest. Ophthalmol. Vis. Sci. 2007;48(13):740.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose:: To evaluate the quantity of Staphylococcus epidermidis cells adhered to different intraocular lens materials.

Methods:: S. epidermidis strain N890074 was incubated for 1 hour or 24 hours on different IOLs: Kelman Duet (silicone), Alcon MZ60BD (PMMA), Alcon SA60AT (acrylate) or Alcon MA60BM (acrylate-metacrilate). Three groups of study: in group I, IOLs were incubated with S. epidermis only; in group II IOLs were immersed in ophthalmic viscosurgical solution (Hydroxypropyl Methylcellulose, Celoftal®) and washed in PBS before incubation with S. epidermidis; in group III, IOLs were incubated in Celoftal® and S. epidermidis (1:1). IOLs were examined by scanning electron microscopy (SEM), to evaluate bacterial biofilm formation in each group.

Results:: Among the four IOLs tested, the lower amount of attached cells was observed on the silicone IOLs. Biofilm production was significantly different between silicone and the rest of lenses at 1 hour and 24 hours (p<0.05). The bacterial populations were no significantly different between IOLs in the three groups at 1 hour of incubation but were significantly different between the group I and group II or III at 24 hours. Between groups II and III were no significant differences were observed at any time point.

Conclusions:: S. epidermidis level of adherence was in the following order: metacrilate (Alcon MA60BM) > acrilate (Alcon SA60AT ) > PMMA (Alcon MZ60) > silicone (Kelman Duet). On the other hand, ophthalmic viscosurgical solution (Hydroxypropyl Methylcellulose, Celoftal®) seems to avoid adherence of cells to the surface lenses.

Keywords: intraocular lens • microscopy: electron microscopy • bacterial disease 

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