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M. Morara, L. Scorolli, A. Meduri, G. Zigiotti, S. Scalinci, P. Greco, R. Meduri; Effect of Basic Fibroblast Growth Factor in Transgenic Mice Corneal Epithelial Healing Process After Excimer Laser Photo-ablation. Invest. Ophthalmol. Vis. Sci. 2007;48(13):782.
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To evaluate the role of prepared Basic Fibroblast Growth Factor eye- drops in corneal epithelial healing of transgenic mice (B6(A)-Rperd12 /J ) after excimer laser photo-ablation.
In our prospective case series, 20 eyes of 10 mice had uneventful bilateral excimer laser photo-ablation. In each mouse, 1 eye received standard topical postoperative therapy with tobramicin, diclofenac and desamethasone eyedrops plusBasic Fibroblast Growth Factor eyedrops 5 µg / 10 µl PBS (3 times a day for 1 week or until corneal re-epithelialization was complete. The fellow eye served as the control and received standard postoperative therapy plus placebo. The mice were monitored daily starting the day after surgery and for 7 days to evaluate the corneal re-epithelialization rate using a video slit-lamp camera with a cobalt blue light. Mean diameter of corneal wounds was measured. Videotaped images were recorded and analyzed by computer planimetry.
All the eyes treated withBasic Fibroblast Growth Factor eyedrops healed completely before day 5 post-surgery, with a mean re-epithelialization time of 92 hours ± 10 (SD); the mean re-epithelialization time was 121 ± 8 hours in eyes receiving placebo (P<.05);. There were no statistically significant differences between the two groups in corneal haze presentation during follow-up (P =.70), perhaps because the time period was too brief (7 days). However, corneal clarity, on slit-lamp bio-microscopy in the study group was greater than in the control group. No side effects or toxic effects were documented.
This data suggests that Basic Fibroblast Growth Factor significantly accelerates epithelial healing after PTK. A further clinical study should be performed to prove the results obtained in this study and the long-term efficacy of Basic Fibroblast Growth Factor to prevent corneal haze.
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