May 2007
Volume 48, Issue 13
ARVO Annual Meeting Abstract  |   May 2007
A Microbial Cellulose Graft for Ocular Surface Defects
Author Affiliations & Notes
  • T. Glonek
    Michael Reese Hospital, Chicago, Illinois
    Family Medicine,
  • D. J. Levinson
    Michael Reese Hospital, Chicago, Illinois
    Internal Medicine,
  • E. Y. Tu
    Ophthalmology, UIC Eye Center, Chicago, Illinois
  • K. R. Kenyon
    Ophthalmology, Harvard Medical School and the Schepens Eye Research Institute, Boston, Massachusetts
  • J. V. Greiner
    Ophthalmology, Harvard Medical School and the Schepens Eye Research Institute, Boston, Massachusetts
  • Footnotes
    Commercial Relationships T. Glonek, None; D.J. Levinson, LCR Woundcare, I; E.Y. Tu, None; K.R. Kenyon, None; J.V. Greiner, None.
  • Footnotes
    Support Res. Ed. Found. MRH Staff, ReFDN 06-05
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 787. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      T. Glonek, D. J. Levinson, E. Y. Tu, K. R. Kenyon, J. V. Greiner; A Microbial Cellulose Graft for Ocular Surface Defects. Invest. Ophthalmol. Vis. Sci. 2007;48(13):787.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose:: To determine whether a bacterial cellulose derived from a Gluconobacter xylinoides subspecies is an effective wound dressing for corneal surface injuries.

Methods:: A G. xylinoides cellulose film was engineered to approximate the size and shape of a 10.0 mm, thin-bandage contact lens. Testing of this microbial cellulose graft material using the pigmented rabbit model (N=6 trials) was conducted following de-epithelialization of the cornea. De-epithelialization was performed by marking the corneal surface with a 9.0 mm trephine and then scraping with a #11 Bard-Parker scalpel blade. Fluorescein staining was used to verify epithelial removal. The control eye was the contralateral eye. Animal care approval was obtained. After placing the microbial cellulose graft material on the corneal surface, a tarsorrhaphy was performed using a single suture. The endpoint was a re-epithelialized corneal surface and/or dissolution of the microbial cellulose graft, as determined by fluorescein staining observed through biomicroscopic examination with a cobalt blue light.

Results:: The microbial cellulose grafts caused no observable discomfort to the rabbits (rubbing, pawing of the lids, other atypical cage behavior). The rabbits re-epithelialized, as measured by no evidence of fluorescein staining, within 24 hr following grafting using the pre-formed cellulose film. Corneas were clear and compact. Rabbits gave no indications of discomfort throughout the 24-hr time-course, and in two instances the grafts appeared to have dissolved.

Conclusions:: Use of the microbial cellulose corneal wound graft promotes corneal healing following de-epithelialization surgery and has potential as a useful material for injured corneal surfaces. These findings using a microbial cellulose material are consistent with those of other on-going studies involving cutaneous wounds, where wound closure was observed to occur at a significantly greater rate of healing in contrast to other standard-of-care treatments.

Keywords: anterior segment • wound healing • regeneration 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.