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S. R. Uhlhorn, D. Borja, N. M. Ziebarth, F. Manns, J.-M. Parel; Optical Coherence Tomography of the Crystalline Lens During Simulated Accommodation. Invest. Ophthalmol. Vis. Sci. 2007;48(13):990.
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© ARVO (1962-2015); The Authors (2016-present)
To obtain cross-sectional images of the crystalline lens and quantify the geometric changes that occur during simulated accommodation.
A time-domain optical coherence tomography (OCT) system has been developed with a scan depth of 10 mm in air and an axial resolution of 12 microns. The OCT system was used to record cross-sectional images of 9 lenses from 5 cynomolgus monkeys during simulated accommodation (4-5 years, <1 day postmortem). A miniature manual lens stretcher (MLS) was developed that simulates accommodation by radially stretching the lens from 8 scleral segments. The lenses are prepared from donated whole globes by gluing the 8 MLS shoes to the sclera of the eye near the lens equator. The posterior pole and cornea are removed from the mounted eye so the lens, zonules, and cilliary body remain intact. The MLS with the mounted lens is placed in a holder containing DMEM solution. OCT scans are recorded for multiple stretching steps, up to 3 steps with approximately 1 mm scleral diameter stretch per step.
OCT images were successfully recorded during lens stretching experiments on 9 lenses from 5 cynomolgus monkeys. Accommodative changes in the lens structure are clearly visible from the images. Qualitatively, it can be seen in the images that as the lens is stretched with the MLS the anterior and posterior lens curvatures decrease and the lens diameter increases. Quantitative measurements of the lens thickness and anterior displacement of the lens during stretching were made from the images. For the 9 eyes, the average thickness change was 0.35 +/- 0.11 mm and the average displacement was 0.32 +/- 0.22 mm.
OCT is an effective tool for visualizing accommodative changes in the crystalline lens. It permits quantitative assessment of lens profiles, thickness, and diameter changes during accommodation in a lens stretching system.Support: Kirschstein NRSA Fellowship NIH F32 EY15630, NIH F31 EY015395, NSF Graduate Fellowship, NIH R01 EY014225, NIH P30 EY014801, Florida Lions Eye Bank, Research to Prevent Blindness, Henri and Flore Lesieur Foundation.
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