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Y. Umino, E. Solessio, R. Hafler, R. Barlow; Duplex Vision in Mice. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1289.
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© ARVO (1962-2015); The Authors (2016-present)
Assess scotopic and photopic vision in mice with behavioral techniques.
We tested visual sensitivity of wildtype (C57BL/6J), rod-less (Gnat2cpfl3) and cone-less (rod transducin α KO) mice by observing their optomotor responses to rotating sinusoidal patterns (Optomotry©) with a double-blind procedure. We measured the spatial and temporal contrast functions over a wide range of illumination (5.6x10-7 cd/m2 to 70 cd/m2).
Mice can see over a 100 million-fold range of illumination using both rods and cones. Rods mediate vision under dim illuminations (< 3x10-4 cd/m2) whereas cones mediate vision with bright level (> 3x10-4 cd/m2). At photopic light levels the contrast sensitivity functions were broadly tuned (0.031 to 0.511 cyc/deg) and band-pass in shape. The contrast sensitivity was nearly invariant over a 30000-fold increase in intensity consistent with Weber’s law. For all spatial frequencies, sensitivity was tuned to the speed of rotation (peak: ~12 deg/sec). Rod-less and wildtype mice had similar sensitivities in the photopic range whereas cone-less mice were completely insensitive. At scotopic light levels, the spatial contrast sensitivity was low-pass in shape, and the spatial frequency range was limited to approximately 0.1-0.2 cyc/deg. Cone-less and wildtype mice had similar sensitivities in the scotopic range, but rod-less mice were insensitive. Remarkably, in the scotopic range visual sensitivity is tuned to the temporal frequency of the rotating pattern rather than to its speed of rotation.
Mice process visual information differently under photopic and scotopic conditions.NIH Grant EY00667, Lions Club of Central New York and Research to Prevent Blindness
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