May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Morphological Damage of Photoreceptor Outer Segments in Rat Retina by Theophylline
Author Affiliations & Notes
  • J.-S. Choi
    Ophthalmology & Visual Science, Catholic Univ Korea Coll of Med, Seoul, Republic of Korea
  • K.-A. Kim
    Ophthalmology & Visual Science, Catholic Univ Korea Coll of Med, Seoul, Republic of Korea
  • Y. Cho
    Agency for Defense Development, Daejeon, Republic of Korea
  • S.-J. Kim
    Agency for Defense Development, Daejeon, Republic of Korea
  • C.-K. Joo
    Ophthalmology & Visual Science, Catholic Univ Korea Coll of Med, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships J. Choi, None; K. Kim, None; Y. Cho, None; S. Kim, None; C. Joo, None.
  • Footnotes
    Support Korea Agency for Defense Development
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 1347. doi:https://doi.org/
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      J.-S. Choi, K.-A. Kim, Y. Cho, S.-J. Kim, C.-K. Joo; Morphological Damage of Photoreceptor Outer Segments in Rat Retina by Theophylline. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1347. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: In order to reveal photoreceptor toxicity as a result of theophylline intoxication, we studied the role of retinal guanylyl cyclase (Ret-GC) and soluble GC (sGC). Theophylline is a non-specific inhibitor of phosphodiesterase (PDE).

Methods:: Sprague dawley rats were used for theophylline toxicity. The drug was administrated by intraperitoneal injection and the toxic effects were observed at 24 hrs after drug administration. To observe photoreceptor injury, light and electron microscopy were both used. And alteration of gene expression was confirmed by western blot analysis and RT-PCR.

Results:: Theophylline induced the degeneration of photoreceptor outer segments 24hrs after drug administration. The toxic effect of theophylline showed dose dependently. And theophylline altered gene expression in photoreceptor. Such as, rhodopsin kinase and recoverin expression are decreased and arrestin and guanylyl cyclase were increased by theophylline. Furthermore, cGMP dependent kinase(cGK) expression level was observed for cGMP mediated cell death by GC activation. The cGK protein and cGMP was increased in this study.

Conclusions:: In this study, theophylline induced morphological change of photoreceptors segments. We also found that theophylline stimulates GC expression and cGMP formation in the retina. Therefore, these results suggest that GC plays a major role in theophylline induced photoreceptor degeneration.

Keywords: photoreceptors • retinal degenerations: cell biology • retina: distal (photoreceptors, horizontal cells, bipolar cells) 
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