May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Interleukin-1ß Alters the Expression of Synaptic Vesicle Associated Proteins and Calcium Signaling in R28 and RGC-5 Cells
Author Affiliations & Notes
  • E. E. Conboy
    Penn State College of Medicine, Hershey, Pennsylvania
    Ophthalmology,
  • S. Shanmugam
    Penn State College of Medicine, Hershey, Pennsylvania
    Cellular and Molecular Physiology,
  • S. F. Abcouwer
    Penn State College of Medicine, Hershey, Pennsylvania
    Surgery,
  • A. J. Barber
    Penn State College of Medicine, Hershey, Pennsylvania
    Ophthalmology,
  • JDRF Diabetic Retinopathy Center
    Penn State College of Medicine, Hershey, Pennsylvania
  • Footnotes
    Commercial Relationships E.E. Conboy, None; S. Shanmugam, None; S.F. Abcouwer, None; A.J. Barber, None.
  • Footnotes
    Support ADA, PA Lions, JDRF Diabetic Retinopathy Research Center
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 1371. doi:https://doi.org/
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      E. E. Conboy, S. Shanmugam, S. F. Abcouwer, A. J. Barber, JDRF Diabetic Retinopathy Center; Interleukin-1ß Alters the Expression of Synaptic Vesicle Associated Proteins and Calcium Signaling in R28 and RGC-5 Cells. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1371. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: It has been shown that Interleukin-1ß (IL-1ß) is present in the inflamed diabetic retina. The aim of this study was to test the hypothesis that IL-1ß alters synaptic protein content and the intracellular calcium response to depolarization in cell culture models of retinal neurons.

Methods:: R28 and RGC-5 cells were grown to sub-confluence and treated with 10ng/ml of IL-1ß for 24 hours. The synaptic vesicle associated protein (VAMP-2) and synapsin were quantified by immunoblotting. The intracellular calcium response to 20mM KCl was measured by live-cell confocal microscopy after incubation with Fluo-4, a cell-permeable calcium indicator.

Results:: Synapsin and VAMP-2 protein contents were significantly decreased after 24 hours of IL-1ß treatment. Concentrations of IL-1ß between 0.1 and 10 ng/ml significantly decreased synapsin protein content. The intracellular Ca2+ response to depolarization was significantly increased by IL-1ß treatment.

Conclusions:: IL-1ß may down-regulate synaptic protein expression in retinal neurons. It may also increase intracellular Ca2+ signaling in response to depolarization. Ca2+signaling is an important regulator of neural function, including synaptic vesicle proteins. These data suggest that inflammatory cytokines, which are elevated in the retina by diabetes, have the potential to alter neuronal function.

Keywords: diabetic retinopathy • synapse • calcium 
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