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P. D. Ramalho, A. Pereira da Silva, P. Pego, C. Thyran, I. Prieto, M. Pires Bicho; Haptoglobin Genotypes May Influence EPO and ACE Activity in Diabetic Retinopathy. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1391.
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© ARVO (1962-2015); The Authors (2016-present)
To study Haptoglobin (Hp) genetic phenotype correlations with EPO and ACE serum expression in diabetes with and without retinopathy.Haptoglobin (α 2- sialoglycoprotein) an acute phase inflammatory serum protein expressed by genetic polymorphism (2 alleles, 3 phenotypes) has been associated to diabetic retinopathy (DR). Erythropoietin (EPO) and Angiotensine converting enzyme (ACE) are upregulated in diabetic retinopathy (PDR).
Hp genotypes 1.1, 2,1, 2.2 were determined using polyacrylamide gel electrophoresis, EPO (mIU/ml) serum levels measured by ELISA, and ACE (mlU/ml) by spectrophotometry in 54 diabetics, 32 with retinopathy. Statistical analysis was carried out using Student "t" test, ANOVA and X2 .
A high number of Hp 2.2 genotypes was observed in diabetics with retinopathy (41,7%) in relation to 2.1 (37.5%) and 1.1 (20,8.7%). In diabetic group without retinopathy the number of Hp 2.1 genotype individuals was greater ( 70,3%, p=0.028). Only in retinopathy group, Hp genotype 2.2 showed high EPO and ACE activity (p=0,06 and p<0,001 respectively) compared to other genotypes.
Haptoglobin 2.2 genotype influencing EPO and ACE activities, could be a risk marker of DR suggesting its inflammatory and neoangiogenic nature, and possibly having therapeutic implications.
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