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R. I. Angunawela, J. Marshall; Inhibition of TGF-beta Induced Corneal Keratocyte Differentiation With the Aloe Vera Derived Sugar, Mannose-6-phosphate. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1488.
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Mannose-6-phosphate (M6P), a macromolecular sugar derived from aloe vera reduces cutaneous scarring by inhibiting transforming growth factor ß (TGF ß) and it effect on fibroblast differentiation to myofibroblasts. We investigated its action on human corneal keratocytes.
Cultured human corneal keratocytes were exposed to TGF ß with or without M6P. Cells were fixed and immunostained for α smooth muscle actin (αSMA) a molecular marker of myofibroblasts. Cells without TGF and M6P were used as controls.
M6P significantly inhibited the effect of TGF ß on corneal keratocytes, suppressing their differentiation to myofibroblasts (p<0.001).
M6P is a cheap and non-toxic inhibitor of TGF ß and a potential agent for preventing haze and regression following corneal refractive surgery.
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