May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Inhibition of TGF-beta Induced Corneal Keratocyte Differentiation With the Aloe Vera Derived Sugar, Mannose-6-phosphate
Author Affiliations & Notes
  • R. I. Angunawela
    Ophthalmology-Rayne Inst, St Thomas Hospital, London, United Kingdom
  • J. Marshall
    Ophthalmology-Rayne Inst, St Thomas Hospital, London, United Kingdom
  • Footnotes
    Commercial Relationships R.I. Angunawela, None; J. Marshall, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 1488. doi:https://doi.org/
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      R. I. Angunawela, J. Marshall; Inhibition of TGF-beta Induced Corneal Keratocyte Differentiation With the Aloe Vera Derived Sugar, Mannose-6-phosphate. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1488. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

Mannose-6-phosphate (M6P), a macromolecular sugar derived from aloe vera reduces cutaneous scarring by inhibiting transforming growth factor ß (TGF ß) and it effect on fibroblast differentiation to myofibroblasts. We investigated its action on human corneal keratocytes.

 
Methods:
 

Cultured human corneal keratocytes were exposed to TGF ß with or without M6P. Cells were fixed and immunostained for α smooth muscle actin (αSMA) a molecular marker of myofibroblasts. Cells without TGF and M6P were used as controls.

 
Results:
 

M6P significantly inhibited the effect of TGF ß on corneal keratocytes, suppressing their differentiation to myofibroblasts (p<0.001).

 
Conclusions:
 

M6P is a cheap and non-toxic inhibitor of TGF ß and a potential agent for preventing haze and regression following corneal refractive surgery.  

 
Keywords: cornea: basic science • refractive surgery • wound healing 
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