May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Gene Expression Profiling of Non-Infectious Uveitis Patients Using Pathway Specific cDNA Microarray Analysis
Author Affiliations & Notes
  • Z. Li
    Lab Immunology, National Eye Inst/NIH, Bethesda, Maryland
  • S. P. Mahesh
    Lab Immunology, National Eye Inst/NIH, Bethesda, Maryland
  • B. Liu
    Lab Immunology, National Eye Inst/NIH, Bethesda, Maryland
  • S. Yeh
    Lab Immunology, National Eye Inst/NIH, Bethesda, Maryland
  • J. Lew
    Lab Immunology, National Eye Inst/NIH, Bethesda, Maryland
  • W. Lim
    Lab Immunology, National Eye Inst/NIH, Bethesda, Maryland
  • G. Levy Clarke
    Lab Immunology, National Eye Inst/NIH, Bethesda, Maryland
  • R. Buggage
    Lab Immunology, National Eye Inst/NIH, Bethesda, Maryland
  • R. B. Nussenblatt
    Lab Immunology, National Eye Inst/NIH, Bethesda, Maryland
  • Footnotes
    Commercial Relationships Z. Li, None; S.P. Mahesh, None; B. Liu, None; S. Yeh, None; J. Lew, None; W. Lim, None; G. Levy Clarke, None; R. Buggage, None; R.B. Nussenblatt, None.
  • Footnotes
    Support supported by the Intramural Research Program of NIH, National Eye Institute
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 1505. doi:https://doi.org/
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    • Get Citation

      Z. Li, S. P. Mahesh, B. Liu, S. Yeh, J. Lew, W. Lim, G. Levy Clarke, R. Buggage, R. B. Nussenblatt; Gene Expression Profiling of Non-Infectious Uveitis Patients Using Pathway Specific cDNA Microarray Analysis. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1505. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: To study gene expression profiling of leukocytes from peripheral blood of patients with non-infectious uveitis.

Methods:: Peripheral blood mononuclear cells (PBMCs) were isolated from 50 patients with clinically characterized non-infectious intermediate uveitis. Total RNA from PBMCs were extracted and quantified. Biotinylated probes were generated from total RNAs by incorporating biotinylated dUTP into synthesized cDNAs using a T7-polymerase linear amplification strategy. RNAs isolated from 20 healthy donors were pooled and served as normal control. Probes were hybridized to a pathway-specific cDNA microarray chip that contains some 400 autoimmune and inflammatory response related genes. Signals for specific binding were recorded by a CCD camera and gene expression profiling was analyzed using GEArray Suite software.

Results:: Despite tremendous heterogeneity of gene expression among patients and normal controls, there were clear differential gene expression patterns comparing those from patients to those from normal control. Although there was more than 80% of overlap of gene expression, some 15% of the genes were differentially expressed among patients compared to the normal donors. Several cytokine, chemokine or chemokine receptor genes were consistently more highly expressed among uveitis patients compared to the normal control. There seems less consistency in terms of down-regulated genes among patients when compared to normal control.

Conclusions:: We show here that pathway specific cDNA microarray can be an efficient and economic strategy to profiling uveitis patients at the genomic level. Although preliminary and needing further confirmation, our data revealed several immune response genes that may be implicated in presumably autoimmune originated non-infectious uveitis.

Clinical Trial:: www.clinicaltrials.gov NCT00357266

Keywords: uveitis-clinical/animal model • gene microarray • autoimmune disease 
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