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D. Shen, R. L. Font, P. LeHoang, M. Zhou, C.-C. Chan; t(11;18) Translocation in Ocular Adnexal Mucosa-Associated Lymphoid Tissue (MALT) Lymphoma. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1584.
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t(11;18)(q21;q21) translocation resulting in the chimaeric transcript of the API2 and MALT1 genes occurs specifically in mucosa associated lymphoid tissue (MALT) lymphoma, which be related with chronic antigenic stimulation provided by infection, such as H. pylori. t(11;18)(q21;q21) translocation has been associated with a tendency to early dissemination and appears to independent to H. pylori. This study is to evaluate t(11;18) translocation and H. pylori in conjunctival and orbital MALT lymphoma.
Nine conjuctival and three orbital MALT lymphoma cases were studied. The lymphoma cells were microdissected from paraffin embedded sections. DNA was extracted for examination of IgH gene rearrangement and presence of UreB and/or VacA genes of H. pylori by PCR. RNA was isolated for expression of t(11;18) translocation by RT-PCR. The primers were API2-S (5'-CCGTGGAAATGGGCTTTAGT-3') and MALT1-AS (5'-AAAGGAGCTTTGAGCTTGGG-3').
All cases illustrated typical morphological features of MALT lymphomas. IgH gene rearrangement was detected in all 12 cases which confirm the diagnosis of MALT lymphoma. DNA of H. pylori was detected in 1/3 orbital and 8/9 conjunctival lymphoma cases. The transcripts of t(11;18) translocation was shown in one obital case, which was negative of H. pylori DNA.
The lower incidence of t(11;18)(q21;q21) translocation in conjunctival and orbital cases compare with that in gastric and pulmonary MALT lymphoma suggest a relatively low grade malgnancy of ocular adnexal MALT lymphoma. H. pylori and t(11;18)(q21;q21) translocation may play important roles in the pathogenesis of ocular adnexal MALT lymphoma independently. Further investigation of translocation-negative and positive MALT lymphoma cases are needed for better understanding of the pathogenesis and treatment of the disease.
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