May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
N-myc Amplification Was Rarely Detected by Fluorescein in situ Hybridization in Retinoblastoma
Author Affiliations & Notes
  • J. Choi
    Biochemistry, Queen's University, Kingston, Ontario, Canada
  • Y. S. Yu
    Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • J. Kim
    Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • D. Kim
    Radiology, Chosun University Hospital, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships J. Choi, None; Y.S. Yu, None; J. Kim, None; D. Kim, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 1596. doi:https://doi.org/
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    • Get Citation

      J. Choi, Y. S. Yu, J. Kim, D. Kim; N-myc Amplification Was Rarely Detected by Fluorescein in situ Hybridization in Retinoblastoma. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1596. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: In retinoblastoma, genetic alteration of N-myc amplification different from the alteration of the RB1 gene on chromosome 13q14 has been described. This study is to determine the frequency of N-myc amplification by fluorescence in situ hybridization method in retinoblastoma

Methods:: The study was prospectively derived from 25 patients who were diagnosed as retinoblastoma (highly progressive retinoblastoma, group 5 in Reese-Ellsworth classification) and went to enucleation. H& E staining was done for pathological examination. Locus specific fluorescence in situ hybridization probes for N-myc gene was used and hundred interphase nuclei were analyzed based on their fluorescence phenotype.

Results:: Although poorly differentiated tumor cells were observed over tumors in all eyes, N-myc amplification was found in only one retinoblastoma.

Conclusions:: N-myc amplification has been regarded as one characteristic of retinoblastoma cell line and an adverse prognostic factor. However, our study indicates that N-myc mutations is not frequently found in retinoblastoma.

Keywords: retinoblastoma • tumors • retina 
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