May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Somatic in utero Gene Delivery to E14 Nrl-/- Photoreceptors Induces Rod Specific Protein Expression
D. C. Chung; T. Rex; V. Lee; W. Tang; Z. Wei; E. Oh; A. Swaroop; J. Bennett
Author Affiliations & Notes
  • D. C. Chung
    FM Kirby Ctr Molecular Ophth, Scheie Eye Institute, Philadelphia, Pennsylvania
  • T. Rex
    FM Kirby Ctr Molecular Ophth, Scheie Eye Institute, Philadelphia, Pennsylvania
  • V. Lee
    FM Kirby Ctr Molecular Ophth, Scheie Eye Institute, Philadelphia, Pennsylvania
  • W. Tang
    FM Kirby Ctr Molecular Ophth, Scheie Eye Institute, Philadelphia, Pennsylvania
  • Z. Wei
    FM Kirby Ctr Molecular Ophth, Scheie Eye Institute, Philadelphia, Pennsylvania
  • E. Oh
    Department of Ophthalmology, University of Michigan, Ann Arbor, Michigan
  • A. Swaroop
    Department of Ophthalmology, University of Michigan, Ann Arbor, Michigan
  • J. Bennett
    FM Kirby Ctr Molecular Ophth, Scheie Eye Institute, Philadelphia, Pennsylvania
  • Footnotes
    Commercial Relationships D.C. Chung, None; T. Rex, None; V. Lee, None; W. Tang, None; Z. Wei, None; E. Oh, None; A. Swaroop, None; J. Bennett, None.
  • Footnotes
    Support NIH RO1 10820, EY11115, Fight for Sight Student Fellowship, NIH K08 EY017024 HIGHWIRE EXLINK_ID="48:5:1691:1" VALUE="EY017024" TYPEGUESS="GEN" /HIGHWIRE -01, Research to Prevent Blindness, the Paul & Evanina Mackall Foundation Trust, the F. M. Kirby Foundation.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 1691. doi:
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      D. C. Chung, T. Rex, V. Lee, W. Tang, Z. Wei, E. Oh, A. Swaroop, J. Bennett; Somatic in utero Gene Delivery to E14 Nrl-/- Photoreceptors Induces Rod Specific Protein Expression. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1691.

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Abstract

Methods:: Transgenic Nrl-/- mice underwent timed pregnancies. At embryonic day 14 (E14), the left subretinal space was injected with an adeno-associated virus encoding the wildtype nrl gene (AAV. CMV2/5.nrl). Non-injected contralateral eyes served as controls. At postnatal day 45 (P45) electroretinograms (ERG) and immunohistochemical analysis for rod-photoreceptor specific proteins were performed.

Results:: Experimental and control eyes exhibited no significant ERG differences. However, histological sections exhibited rhodopsin and transducin positive photoreceptors cells.

Conclusions:: In utero gene delivery can alter isolated photoreceptor cell differentiation from a cone pathway to a rod pathway evidenced by the expression of rod-specific proteins. Earlier delivery may increase the number of cells with a rod photoreceptor fate. Future visual behavioral analysis will also evaluate rod cell function.<br /

Keywords: development • photoreceptors • gene transfer/gene therapy 
© 2007, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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