May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Somatic in utero Gene Delivery to E14 Nrl-/- Photoreceptors Induces Rod Specific Protein Expression
Author Affiliations & Notes
  • D. C. Chung
    FM Kirby Ctr Molecular Ophth, Scheie Eye Institute, Philadelphia, Pennsylvania
  • T. Rex
    FM Kirby Ctr Molecular Ophth, Scheie Eye Institute, Philadelphia, Pennsylvania
  • V. Lee
    FM Kirby Ctr Molecular Ophth, Scheie Eye Institute, Philadelphia, Pennsylvania
  • W. Tang
    FM Kirby Ctr Molecular Ophth, Scheie Eye Institute, Philadelphia, Pennsylvania
  • Z. Wei
    FM Kirby Ctr Molecular Ophth, Scheie Eye Institute, Philadelphia, Pennsylvania
  • E. Oh
    Department of Ophthalmology, University of Michigan, Ann Arbor, Michigan
  • A. Swaroop
    Department of Ophthalmology, University of Michigan, Ann Arbor, Michigan
  • J. Bennett
    FM Kirby Ctr Molecular Ophth, Scheie Eye Institute, Philadelphia, Pennsylvania
  • Footnotes
    Commercial Relationships D.C. Chung, None; T. Rex, None; V. Lee, None; W. Tang, None; Z. Wei, None; E. Oh, None; A. Swaroop, None; J. Bennett, None.
  • Footnotes
    Support NIH RO1 10820, EY11115, Fight for Sight Student Fellowship, NIH K08 EY017024 HIGHWIRE EXLINK_ID="48:5:1691:1" VALUE="EY017024" TYPEGUESS="GEN" /HIGHWIRE -01, Research to Prevent Blindness, the Paul & Evanina Mackall Foundation Trust, the F. M. Kirby Foundation.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 1691. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      D. C. Chung, T. Rex, V. Lee, W. Tang, Z. Wei, E. Oh, A. Swaroop, J. Bennett; Somatic in utero Gene Delivery to E14 Nrl-/- Photoreceptors Induces Rod Specific Protein Expression. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1691. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Methods:: Transgenic Nrl-/- mice underwent timed pregnancies. At embryonic day 14 (E14), the left subretinal space was injected with an adeno-associated virus encoding the wildtype nrl gene (AAV. CMV2/5.nrl). Non-injected contralateral eyes served as controls. At postnatal day 45 (P45) electroretinograms (ERG) and immunohistochemical analysis for rod-photoreceptor specific proteins were performed.

Results:: Experimental and control eyes exhibited no significant ERG differences. However, histological sections exhibited rhodopsin and transducin positive photoreceptors cells.

Conclusions:: In utero gene delivery can alter isolated photoreceptor cell differentiation from a cone pathway to a rod pathway evidenced by the expression of rod-specific proteins. Earlier delivery may increase the number of cells with a rod photoreceptor fate. Future visual behavioral analysis will also evaluate rod cell function.<br /

Keywords: development • photoreceptors • gene transfer/gene therapy 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×