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T. Mimura, T. Onguchi, T.-I. Kim, J.-H. Chang, D. T. Azar; The Effect of MT1-MMP on Decorin, The Anti-Angiogenic Proteoglycan, In Cornea. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1703. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
Decorin is a small chondroitin sulfate proteoglycan. It is localized to the corneal stroma and may play a regulatory role in angiogenesis. MT1-MMP has been shown to be an important angiogenic enzyme present in the cornea. The aim of this study is to determine the effect of MT1-MMP on Decorin processing and degradation.
Decorin is incubated with varying concentrations of human recombinant MT1-MMP at different time points (from 0 to 20 hours), with different Zn2+ and Ca2+ concentrations and pH. The degradation pattern of Decorin was analyzed by Western blot analysis.
Decorin is processed by MT1-MMP in vitro in the presence of Zn2+ and Ca2+. The kinetics of Decorin proteolytic processing by MT1-MMP is regulated by MT1-MMP concentration and incubation time. Decorin proteolysis by MT1-MMP is enhanced at lower pH (6.5) when compared to that of physiologic pH (7.4).
Decorin is a substrate of MT1-MMP in vitro. The angiogenic role of MT1-MMP may include its ability to cleave Decorin.
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