May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
The Effect of MT1-MMP on Decorin, The Anti-Angiogenic Proteoglycan, In Cornea
Author Affiliations & Notes
  • T. Mimura
    The Schepens Eye Research Institute, Harvard Medical School, Boston, Massachusetts
    Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois
  • T. Onguchi
    Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois
  • T.-I. Kim
    The Schepens Eye Research Institute, Harvard Medical School, Boston, Massachusetts
  • J.-H. Chang
    The Schepens Eye Research Institute, Harvard Medical School, Boston, Massachusetts
    Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois
  • D. T. Azar
    The Schepens Eye Research Institute, Harvard Medical School, Boston, Massachusetts
    Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois
  • Footnotes
    Commercial Relationships T. Mimura, None; T. Onguchi, None; T. Kim, None; J. Chang, None; D.T. Azar, None.
  • Footnotes
    Support NIH Grant EY10101 (DTA), and EY14048 (JHC)
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 1703. doi:https://doi.org/
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    • Get Citation

      T. Mimura, T. Onguchi, T.-I. Kim, J.-H. Chang, D. T. Azar; The Effect of MT1-MMP on Decorin, The Anti-Angiogenic Proteoglycan, In Cornea. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1703. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: Decorin is a small chondroitin sulfate proteoglycan. It is localized to the corneal stroma and may play a regulatory role in angiogenesis. MT1-MMP has been shown to be an important angiogenic enzyme present in the cornea. The aim of this study is to determine the effect of MT1-MMP on Decorin processing and degradation.

Methods:: Decorin is incubated with varying concentrations of human recombinant MT1-MMP at different time points (from 0 to 20 hours), with different Zn2+ and Ca2+ concentrations and pH. The degradation pattern of Decorin was analyzed by Western blot analysis.

Results:: Decorin is processed by MT1-MMP in vitro in the presence of Zn2+ and Ca2+. The kinetics of Decorin proteolytic processing by MT1-MMP is regulated by MT1-MMP concentration and incubation time. Decorin proteolysis by MT1-MMP is enhanced at lower pH (6.5) when compared to that of physiologic pH (7.4).

Conclusions:: Decorin is a substrate of MT1-MMP in vitro. The angiogenic role of MT1-MMP may include its ability to cleave Decorin.

Keywords: cornea: basic science 
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