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A. S. Solomon, M. Blank, Y. Shoenfeld; Can IVIG Inhibit Formation of Tumor by Inseminated Cells in an Eye Model?. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1721. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the inhibitory potential of IVIG (Intravenous Ig) on inseminated tumor cells by preventing tumor formation
An animal eye model was used in the presented study.The cornea of albino New Zealand laboratory rabbit was used as site of tumor cells insemination.Under general anesthesia according to ARVO Rules for Animal Care and under Animal Care Committe Approval a concentration of 5,000 tumor cells (CT26) per micro liter was injected sub epithelium of cornea close to the corneal border.A volume of 2 micro liters of cells was injected in the right eye of each rabbit. Three groups of rabbits were included , three rabbits in each one. The first group was inseminated with cells.only.The second group was injected in adition to the cells with 2 micro liter of PBS .The third group was injected in adition to the tumor cells with 2 micro liter of IVIG . The injection of PBS and IVIG was done at a two days interval and a total of three injections. Follow up was done by slit lamp examination before each injection.The end of the study was at 10 days from the start.
The cornea inseminated with tumor cells formed a rich vascular bed and formed a well defined tumor SOL covered by vascular net.The same was for the PBS group.The IVIG cornea presented a poor vascular net and at 10 days from the injection the tumor cells could not be observed .
IVIG has an inhibitory effect on the development and organisation of inseminated tumor cells in the cornea.It may have a topical application for inhibition or destruction of local intra ocular tumors.
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