May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Soluble VEGF Receptor-1 Restores Corneal Avascularity in Corn1 and Pax6+/- Mice
Author Affiliations & Notes
  • A. Takeda
    Dept of Ophthal and Visual Sci, University of Kentucky, Lexington, Kentucky
  • K. Yamada
    Dept of Ophthal and Visual Sci, University of Kentucky, Lexington, Kentucky
  • M. Nozaki
    Dept of Ophthal and Visual Sci, University of Kentucky, Lexington, Kentucky
  • B. Raisler
    Dept of Ophthal and Visual Sci, University of Kentucky, Lexington, Kentucky
  • R. Albuquerque
    Dept of Ophthal and Visual Sci, University of Kentucky, Lexington, Kentucky
  • J. Baffi
    Dept of Ophthal and Visual Sci, University of Kentucky, Lexington, Kentucky
  • M. Kleinman
    Dept of Ophthal and Visual Sci, University of Kentucky, Lexington, Kentucky
  • B. K. Ambati
    Dept of Ophthal and Visual Sci, University of Kentucky, Lexington, Kentucky
  • J. Ambati
    Dept of Ophthal and Visual Sci, University of Kentucky, Lexington, Kentucky
  • Footnotes
    Commercial Relationships A. Takeda, None; K. Yamada, None; M. Nozaki, None; B. Raisler, None; R. Albuquerque, None; J. Baffi, None; M. Kleinman, None; B.K. Ambati, None; J. Ambati, None.
  • Footnotes
    Support The Japan Society for the Promotion of Science
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 1733. doi:https://doi.org/
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    • Get Citation

      A. Takeda, K. Yamada, M. Nozaki, B. Raisler, R. Albuquerque, J. Baffi, M. Kleinman, B. K. Ambati, J. Ambati; Soluble VEGF Receptor-1 Restores Corneal Avascularity in Corn1 and Pax6+/- Mice. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1733. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: We reported that soluble VEGF receptor-1 (sflt-1) expressed in corneal epithelium binds VEGF-A and maintains corneal avascularity. Although corn1 and Pax6+/– mouse corneas are known to spontaneously vascularize, the underlying mechanisms are unclear. We studied corneal expression of sflt-1 in these mutant mice and in Pax6+/– patients with aniridia associated neovascularization. We also tested the effect of exogenous sflt-1 administration on these mutant corneas.

Methods:: sflt-1 expression in the corneas of A/J, corn1, Pax6+/+, and Pax6+/– mice was determined by western blotting and in aniridic corneas by immunohistochemistry. sflt-1/Fc or IgG1-Fc protein was injected into the corneas of corn1 and Pax6+/– mice. Cornea flat mounts were stained with CD31-FITC to detect blood vessels.

Results:: The avascular corneas of A/J and Pax6+/+ mice expressed sflt-1 whereas corn1 and Pax6+/– corneas did not. Enforced expression of recombinant sflt-1 restored corneal avascularity in these mutant mice. The corneas of patients with aniridia were deficient in sflt-1 compared to normal human corneas.

Conclusions:: Dysregulation of sflt-1 is involved in these models of spontaneous corneal neovascularization and administration of sflt-1 can be effective in treating this blinding condition.

Keywords: cornea: basic science • neovascularization 
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