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S. Kaliappan, V. V. Lyzogubov, R. G. Tytarenko, N. S. Bora, P. S. Bora; Alcohol and Nicotine Feeding Increases the Severity of Choroidal Neovascularization in Rats. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1759.
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To investigate the effect of alcohol and nicotine consumption on the pathology of choroidal neovascularization (CNV) in rats after laser-photocoagulation.
Male Brown Norway rats were divided into four groups. The animals in all four groups received the regular diet and CNV was induced by laser photocoagulation (using the Argon red laser). After laser, group I received alcohol (8 g/Kg body weight), group II received nicotine (200µg/ml) and group III was fed with a combination of alcohol and nicotine. The group IV that was fed with water served as the control group. The animals in all groups were sacrificed 4 weeks after the alcohol and nicotine feeding, perfused with FITC-dextran and eyes were enucleated. RPE-choroidal -scleral flat mounts were stained with an anti-elastin (CY3 conjugated) antibody. The incidence of CNV was determined by confocal microcopy and the size of CNV complex was measured using Image Pro-Plus software. Paraffin embedded sections of liver, lung, spleen, pancreas and kidney were used for toxicology study.
Confocal microscopic analysis showed a 3.4 fold increase in CNV complex size in alcohol fed rats compared to water fed controls. Our results further demonstrated that there was a 2 fold increase in CNV complex size in rats treated with nicotine as compared to the control group while the combination of alcohol and nicotine treatment resulted in 3.5 fold increase in CNV complex size.
Our results demonstrate that the alcohol and nicotine treatment increases the severity of CNV in rats. These results suggest that alcohol and nicotine consumption may predispose to AMD.
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