May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Anesthesia Protocol for Fluorescein Angiography in Non-Human Primates
Author Affiliations & Notes
  • J. G. Conrath
    Ophthalmology, Hopital de la Timone, Marseille, France
  • L. Federici
    Ophthalmology, Hopital de la Timone, Marseille, France
  • M. Jaquier
    CNRS, Marseille, France
  • L. Hoffart
    Ophthalmology, Hopital de la Timone, Marseille, France
  • F. Chavane
    CNRS / Team DyVA, Marseille, France
  • G. Masson
    CNRS / Team DyVA, Marseille, France
  • B. Ridings
    Ophthalmology, Hopital de la Timone, Marseille, France
  • Footnotes
    Commercial Relationships J.G. Conrath, None; L. Federici, None; M. Jaquier, None; L. Hoffart, None; F. Chavane, None; G. Masson, None; B. Ridings, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 1779. doi:https://doi.org/
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    • Get Citation

      J. G. Conrath, L. Federici, M. Jaquier, L. Hoffart, F. Chavane, G. Masson, B. Ridings; Anesthesia Protocol for Fluorescein Angiography in Non-Human Primates. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1779. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: To describe a general anesthesia protocol used for non-human primates in research in ophthalmology, specifically to inhibit vomiting often observed during intravenous fluorescein fundus angiography.

Methods:: Baboons were used in an experimental protocol involving laser-induced subretinal neovascularization. Fluorescein angiography was practiced under general anesthesia. In order to inhibit vomiting, animals received 0.5mg/kg metoclopramide intra-muscularly 45 minutes before general anesthesia. Medetomidine hydrochloride (0.04 mg/kg) and tiletamine/zolazepam (2 mg/kg) was used for general anesthesia and then atropine sulfate was administered at 0;04 mg/kg. Anesthesia was maintained by injection of diazepam (0.25 mg/kg).

Results:: This protocol allowed realization of fluorescein angiography in optimal conditions, with no vomiting, retching or hypersalivation.

Conclusions:: Often it is difficult to obtain a full sequence of fluorescein angiograms in non-human primates as vomiting may occur between the first and fifth minute post-injection. Our protocol appears to limit this inconvenience.

Keywords: choroid: neovascularization • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) 
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