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J. G. Conrath, L. Federici, M. Jaquier, L. Hoffart, F. Chavane, G. Masson, B. Ridings; Anesthesia Protocol for Fluorescein Angiography in Non-Human Primates. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1779. doi: https://doi.org/.
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To describe a general anesthesia protocol used for non-human primates in research in ophthalmology, specifically to inhibit vomiting often observed during intravenous fluorescein fundus angiography.
Baboons were used in an experimental protocol involving laser-induced subretinal neovascularization. Fluorescein angiography was practiced under general anesthesia. In order to inhibit vomiting, animals received 0.5mg/kg metoclopramide intra-muscularly 45 minutes before general anesthesia. Medetomidine hydrochloride (0.04 mg/kg) and tiletamine/zolazepam (2 mg/kg) was used for general anesthesia and then atropine sulfate was administered at 0;04 mg/kg. Anesthesia was maintained by injection of diazepam (0.25 mg/kg).
This protocol allowed realization of fluorescein angiography in optimal conditions, with no vomiting, retching or hypersalivation.
Often it is difficult to obtain a full sequence of fluorescein angiograms in non-human primates as vomiting may occur between the first and fifth minute post-injection. Our protocol appears to limit this inconvenience.
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