May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Culture Medium Affects Adhesive Behavior of Corneal Stromal Cells on Chitosan-Coated Surface and Tissue Culture Plate
Y.-H. Chen; I.-J. Wang; T.-H. Young
Author Affiliations & Notes
  • Y.-H. Chen
    Institution of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan
    Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan
  • I.-J. Wang
    Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan
  • T.-H. Young
    Institution of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan
  • Footnotes
    Commercial Relationships Y. Chen, None; I. Wang, None; T. Young, None.
  • Footnotes
    Support NSC 95-2314-B-002-149-MY3
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 1874. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Culture Medium Affects Adhesive Behavior of Corneal Stromal Cells on Chitosan-Coated Surface and Tissue Culture Plate
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      Y.-H. Chen, I.-J. Wang, T.-H. Young; Culture Medium Affects Adhesive Behavior of Corneal Stromal Cells on Chitosan-Coated Surface and Tissue Culture Plate. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1874.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Purpose:: To determine the difference of cell adhesive molecules between adhesive and spheroidic corneal stromal cells on chitosan and tissue-culture plate in two different medium.

Methods:: Collagenase-digested bovine corneal stromal cells were seeded onto chitosan-coated plate (CM) and tissue culture plate (TCPS) with neural cell cultured medium (NCM) , which containing DMEM/F12, B27 supplement, EGF 10ng/ml, and bFGF 20ng/ml, or DMEM with 10% horse serum(HS). Cell viability of each group was determined by MTT assay. The mRNA of Keratin 3 (K3), keratocan, alpha-smooth muscle actin(alpha-SMA), and PAX6 were recognized by RT-PCR. To compare the cell adhesive force, RT-PCR was used to detect the cell adhesive molecules CD34, N-cadherin, cadherin 11, alpha5 integrin, and alpha v integrin.

Results:: Corneal stromal cell seeding on CM with DMEM/10% HS (C/H group) formed suspended cell-sphereoids. Moreover, cells attached to the TCPS with DMEM/10HS (T/H group) were dendritic shape. Cells in the NCM showed totally inverse adhesive property. Cells seeding on the CM with NCM (C/N group) surprisingly attached to the surface and showed morphologically smoother cell boundary than T/H group. Cells in NCM seeding on TCPS (T/N group) formed cell-spheroids and some of spheroids attached to the plates. To determine the cell activity, the results of MTT assay indicated that the attached cells ( T/H and C/N groups) showed higher mitochondrial activity than spheroids cells (C/H and T/N groups). RT-PCR results showed that cells under all four conditions presented keratocan-positive, alphaSMA-negative, and K3-negative phenotypes. In addition, PAX6 presented light positive for T/N and C/N groups and negative for T/H and C/H group. About the cell adhesive molecules, all groups expressed alph v integrin and cadhrin 11. In addition, only T/H and T/N groups showed CD34 positive and only C/N group expressed alpha 5 integrin negative.

Conclusions:: Cell adhesive property and cell viability are different under different culture condition.

Keywords: cornea: stroma and keratocytes • cell adhesions/cell junctions 
© 2007, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept