May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Direct Observation of Multiple Functional Intrinsic Signals Induced by Patterned Visible Light Stimuli
Author Affiliations & Notes
  • D. Itzhaki
    Optical Imaging Ltd., Rehovot, Israel
  • D. R. Nelson
    Optical Imaging Ltd., Rehovot, Israel
  • A. Grinvald
    Optical Imaging Ltd., Rehovot, Israel
    Neurobiology, Weizmann Institute of Science, Rehovot, Israel
  • Footnotes
    Commercial Relationships D. Itzhaki, Optical Imaging, Ltd., E; D.R. Nelson, Optical Imaging, Ltd., I; Optical Imaging, Ltd., E; A. Grinvald, Optical Imaging, Ltd., I; Optical Imaging, Ltd., E.
  • Footnotes
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Investigative Ophthalmology & Visual Science May 2007, Vol.48, 1952. doi:https://doi.org/
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    • Get Citation

      D. Itzhaki, D. R. Nelson, A. Grinvald; Direct Observation of Multiple Functional Intrinsic Signals Induced by Patterned Visible Light Stimuli. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1952. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: Retinal reflectance changes in response to stimulation carry information about metabolic processes underlying light responses in the retina. The Retinal Function Imager (RFI, Optical Imaging, Ltd.) was used to non-invasively image induced reflectance changes in monkey and human retina.

Methods:: The RFI measures functional signals in retinal image series recorded at chosen wavelengths. We measured changes in retinal reflectance during 12-second trials with two visual stimulus test patterns and blank as a control. A patterned stimulus flashed once or at 4-30Hz during recording in infrared from eyes of anesthetized monkeys or awake human.

Results:: Multiple intrinsic functional signals were clearly resolved, varying in amplitude and duration and yielding a change of up to 0.5% in retinal reflectance. The extent of the spatially specific response matched the stimulus patterns. Stimulus wavelength, frequency, duration and intensity all strongly affected the signal.

Conclusions:: The measured optical signals provide information about activity-dependent metabolic processes in the retina. The localized signals also provide an objective indicator of retinal function, suggesting clinical applications.

Keywords: retina • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • photoreceptors 
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