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E. E. Gabison, E. Huet, B. Valée, D. Szul, F. Verrecchia, T. Hoang-Xuan, S. Menashi; Role of EMMPRIN (cd147), An Inducer of Matrix Metalloproteinases Synthesis During Corneal Remodelling. Invest. Ophthalmol. Vis. Sci. 2007;48(13):1960. doi: https://doi.org/.
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We previously described EMMPRIN, the metalloproteinase inducer, in the cornea and demonstrated its up-regulation during corneal healing process (E. E. Gabison et al. Am J Pathol 2005). In this study we investigated its role during corneal remodelling.
EMMPRIN expression was analyzed in normal and ulcerated human corneas, as well as in stromal cells in culture using confoncal microscopy, immuno-blots and real-time PCR.
In normal cornea EMMPRIN was predominantly expressed in the epithelium but was markedly induced in the anterior stroma of ulcerated corneas. This coincided with MMP-2 induction which colocalized with EMMPRIN at the epithlio-stromal boundary. In an in-vitro coculture system we demonstrated the induction and colocalization of EMMPRIN and MMP-2 in the fibroblasts at the interface with epithelial cells. Direct contact of fibroblasts with EMMPRIN-containing purified epithelial cell membranes also induced MMP-1, MMP-2 and EMMPRIN and this was inhibited by a blocking anti-EMMPRIN antibody. Additionnaly, EMMPRIN and MMP-2 were also induced by TGF-b in corneal fibroblasts in culture
EMMPRIN up-regulation in the stroma during corneal wound healing and its modulation by TGFb and cell-cell interactions suggest an important role in corneal remodelling
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