Purchase this article with an account.
C. Strohmaier, R. Werkmeister, G. Grabner, B. Tockner, B. Bogner, C. Runge, A. Bachernegg, L. Schmetterer, H. A. Reitsamer; Microscope Based Laser Doppler Flowmeter for Rodent Use. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2265.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To develop a non-invasive microscope based laser Doppler blood flowmeter for rodent use.
Based on the theory of Bonner and Nossal, a number proportional to blood flow in capillaries can be calculated as the product of mean velocity of erythrocytes and the concentration of moving erythrocytes in the tissue. In our setup, both values are derived from processing the Doppler shifted signal of a laser beam (811 nm) that is directed to the tissue of interest. The laser diode as well as the detector are mounted on a microscope, one eye piece has a CCD camera attached to check the illumination site of the laser. The analysis is done by software based on the LabView developing environment.
Previous pharmacological studies on the effect of L-NAME on choroidal blood flow were repeated in mice, rats and rabbits. Measurements were performed using a PeriFlux System 5000 flowmeter with a standard probe (411) as a control device. The measurements generated the same results as found in previous studies using L-NAME with good correlation between the flow values of the Periflux flowmeter and the device presented in this study. In mice and rats the Doppler shifts are generated by the retinal and the choroidal circulation, whereas in rabbits the signal was generated by the choroidal circulation only.
The presented microscope provides a valid, non-invasive measurement of blood flow blood flow in ocular tissues.
This PDF is available to Subscribers Only