May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
The Change of Prostaglandins,and Cysteinyl-leukotriene Concentrations in the Tears of Patients With Severe Allergic Conjunctivitis
Author Affiliations & Notes
  • A. Iga Rashi
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • N. Okada
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Y. Satake
    Ophthalmology, Tokyo Dental College, Ichikawa, Japan
  • M. Dogru
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • K. Fukagawa
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • J. Shimazaki
    Ophthalmology, Tokyo Dental College, Ichikawa, Japan
  • K. Tsubota
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • H. Fujishima
    Ophthalmology, Keio University School of Medicine, Tokyo, Japan
  • Footnotes
    Commercial Relationships A. Iga Rashi, None; N. Okada, None; Y. Satake, None; M. Dogru, None; K. Fukagawa, None; J. Shimazaki, None; K. Tsubota, None; H. Fujishima, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 2310. doi:
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      A. Iga Rashi, N. Okada, Y. Satake, M. Dogru, K. Fukagawa, J. Shimazaki, K. Tsubota, H. Fujishima; The Change of Prostaglandins,and Cysteinyl-leukotriene Concentrations in the Tears of Patients With Severe Allergic Conjunctivitis. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2310.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: Prostaglandins(PGs)and cysteinyl-leukotrienes(Cys-LTs) have been reported to mediate various inflammatory responses. However, studies detailing the levels of PGs and Cys-LTs in tears of patients with atopic keratoconjunctivitis (AKC) and vernal keratoconjunctivitis (VKC) have not yet been extensively reported. In this study, we examined the concentration of PGD2 , PGJ2, PGE2,and Cysteinyl-Leukotriene in tears from patients with severe allergic conjunctivitis.

Methods:: Tear samples were collected from normal control subjects and from patients with VKC and AKC. 20 µL of tears were collected by capillary tubes; samples were centrifuged 10000rpm for 5 minutes. The supernatants were then stored at -20°C until concentration measurements by ELISA and EIA.The concentrations of PGD2(12eyes),PGE2(16eyes),PGJ2(10eyes),Cysteinyl-Loukotrienes(16eyes) were measured using ELISA and EIA.The concentrations were then compared between patients with AKC, VKC and normal control subjects using the t-test for statistical comparisons.Informed consents were obtained from all patients.This study followed the Tenets of the Declaration of Helsinki.

Results:: The concentrations of PGD2, PGE2, PGJ2, and cysteinyl-Leukotriene were found to be significantly higher (P<0.05) in patients with allergic conjunctivitis than in normal control subjects. However there were no statistically significant differences between patients with AKC and VKC.

Conclusions:: Both PGs and LTs appear to significantly contribute to the inflammatory response and development of severe allergic conjunctivitis.

Keywords: conjunctivitis • cornea: tears/tear film/dry eye • clinical laboratory testing 
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