May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Anti-Oxidization Effects of Proteasome Beta-Subunit Overexpression in Lens Epithelial Cells
Author Affiliations & Notes
  • X. Liu
    Zhongshan Ophthalmic Center, Sun Yat-sen Univ Medical Sci, Guangzhou, China
  • T. Zhang
    Zhongshan Ophthalmic Center, Sun Yat-sen Univ Medical Sci, Guangzhou, China
  • Y. Liu
    Zhongshan Ophthalmic Center, Sun Yat-sen Univ Medical Sci, Guangzhou, China
  • Footnotes
    Commercial Relationships X. Liu, None; T. Zhang, None; Y. Liu, None.
  • Footnotes
    Support grant1:NSFC No.30070802 (China); grant2Science Foundation of Guangzhou No.2002Z1-Z0141 (China)
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 2439. doi:
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    • Get Citation

      X. Liu, T. Zhang, Y. Liu; Anti-Oxidization Effects of Proteasome Beta-Subunit Overexpression in Lens Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2439.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: To evaluate the potential protective role against oxidative stress of overexpression of the proteasome catalytic subunit beta 5 in lens epithelial cells.

Methods:: Cells from the human lens epithelial cell line SRA01/04 (LECs) were stably transfected either with a plasmid expressing the proteasome catalytic subunit beta5 or with an empty plasmid. Beta5-expressing LECs and controls were analyzed for the expression of beta1, beta2, beta5 and alpha1, alpha2, alpha6 proteasome subunits; chymotrypsin-like (CT-L) and peptidylglutamyl-peptide hydrolase (PGPH) catalytic activities; as well as for the accumulation of carbonylated proteins, rates of cell viability, and apoptosis after oxidative stress.

Results:: Stable expression of the beta5 proteasome subunit resulted in increased expression of the other beta catalytic subunits, increased CT-L and PGPH proteasome activities, and increased resistance to accumulation of carbonylated proteins and cell death after oxidative stress.

Conclusions:: The proteasome can be genetically "up-regulated" in lens epithelial cells by overexpression of the beta1 and beta5 catalytic subunit. The resulting increase in proteasome activity leads to a decrease in the accumulation of oxidized proteins and enhanced cell survival following oxidative stress.

Keywords: antioxidants • oxidation/oxidative or free radical damage • cataract 
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