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J. Yaung, R. Kannan, C. Spee, P. G. Sreekumar, D. R. Hinton; Regulation of -Crystallins in Cobalt Chloride-Induced Retinal Hypoxia and Degeneration. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2508.
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We previously showed that lack of crystallins cause cell death in RPE. The aim of the present study is to further elucidate the mechanism of retinal degeneration and the role of crystallins under chemically-induced hypoxic conditions.
Retinal degeneration is chemically induced by cobalt-chloride intravitreal injection. Hematoxylin/eosin (H&E), TUNEL, αA- and αB-crystallin staining followed after 24 hours, 96 hours, 1week, and 2 weeks post-injection. In separate experiments in RPE in vitro, the effect of cobalt chloride on αB-crystallin expression was determined by real time PCR, Western, and confocal microscopy. Subcellular distribution of αB-crystallin in the nucleus, mitochondria, and cytosol was examined by fractionation.
H&E and TUNEL results confirmed retinal degeneration with CoCl2 injection with accompanying changes in the expression of αA- and αB-crystallins. Cultured RPE studies showed a decrease in αB-crystallin expression with increasing CoCl2 treatment. Subcellular distribution of αB-crystallin in the nucleus, mitochondria, and cytosol showed differential expression within each compartment.
α-Crystallins may play an important role in maintenance of the pathophysiology of retina and RPE cells.
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