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C.-C. Lai, W.-C. Wu, N.-K. Wang, L. Yeung, Y.-P. Chen, Y.-S. Hwang, K.-J. Chen, L.-H. Chuang, T.-L. Chen; The Toxic Gradient of Staining Materials for Macular Surgery in Retinal Cell Culture. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2539.
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To evaluate the toxic gradient of staining materials used in dye-assisted internal limiting membrane peeling for macular surgery.
Different concentrations of staining materials included indocyanine green (ICG), trypan blue (TB) and triamcinolone acetonide (TA) were prepared. The concentrations for cell culture were, ICG (5, 1, 0.5 mg/ml), TB (1.5, 0.6, 0.2 mg/ml) and TA (40, 8 mg/ml with or without preservative), which had been applied in clinical patients. Cultured human RPE cells or retinal ganglion cells were exposed to various concentrations of pre-prepared solutions of staining materials for 5, 20, 60 minutes and 6 hours. The toxic effect of staining materials in cultured cells was evaluated by mitochondrial dehydrogenase assay.
In the RPE cell culture, the survival rate of cells in TA (40mg/ml with preservative) were the lowest in different time intervals.Cells cultured with TA (8 mg/ml without preservative) had hihgest survival rate. In the ganglion cell culture, the ICG (5 mg/mL) was the most toxic dye and TA (8 mg/ml without preservative) showed least toxic to cells. The toxicty of dyes was dose and time-dependent.
In this study, TA (8 mg/ml without preservative) is less toxic in cell culture when compare to the other clinical staining materials.
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