May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
The Toxic Gradient of Staining Materials for Macular Surgery in Retinal Cell Culture
Author Affiliations & Notes
  • C.-C. Lai
    Ophthalmology, Chang Gung Memorial Hospital, Kwei-Shan, Taiwan
  • W.-C. Wu
    Ophthalmology, Chang Gung Memorial Hospital, Kwei-Shan, Taiwan
  • N.-K. Wang
    Ophthalmology, Chang Gung Memorial Hospital, Kwei-Shan, Taiwan
  • L. Yeung
    Ophthalmology, Chang Gung Memorial Hospital, Keelung, Taiwan
  • Y.-P. Chen
    Ophthalmology, Chang Gung Memorial Hospital, Kwei-Shan, Taiwan
  • Y.-S. Hwang
    Ophthalmology, Chang Gung Memorial Hospital, Kwei-Shan, Taiwan
  • K.-J. Chen
    Ophthalmology, Chang Gung Memorial Hospital, Kwei-Shan, Taiwan
  • L.-H. Chuang
    Ophthalmology, Chang Gung Memorial Hospital, Keelung, Taiwan
  • T.-L. Chen
    Ophthalmology, Chang Gung Memorial Hospital, Kwei-Shan, Taiwan
  • Footnotes
    Commercial Relationships C. Lai, None; W. Wu, None; N. Wang, None; L. Yeung, None; Y. Chen, None; Y. Hwang, None; K. Chen, None; L. Chuang, None; T. Chen, None.
  • Footnotes
    Support NMRPG450061, Taiwan
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 2539. doi:
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    • Get Citation

      C.-C. Lai, W.-C. Wu, N.-K. Wang, L. Yeung, Y.-P. Chen, Y.-S. Hwang, K.-J. Chen, L.-H. Chuang, T.-L. Chen; The Toxic Gradient of Staining Materials for Macular Surgery in Retinal Cell Culture. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2539.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose:: To evaluate the toxic gradient of staining materials used in dye-assisted internal limiting membrane peeling for macular surgery.

Methods:: Different concentrations of staining materials included indocyanine green (ICG), trypan blue (TB) and triamcinolone acetonide (TA) were prepared. The concentrations for cell culture were, ICG (5, 1, 0.5 mg/ml), TB (1.5, 0.6, 0.2 mg/ml) and TA (40, 8 mg/ml with or without preservative), which had been applied in clinical patients. Cultured human RPE cells or retinal ganglion cells were exposed to various concentrations of pre-prepared solutions of staining materials for 5, 20, 60 minutes and 6 hours. The toxic effect of staining materials in cultured cells was evaluated by mitochondrial dehydrogenase assay.

Results:: In the RPE cell culture, the survival rate of cells in TA (40mg/ml with preservative) were the lowest in different time intervals.Cells cultured with TA (8 mg/ml without preservative) had hihgest survival rate. In the ganglion cell culture, the ICG (5 mg/mL) was the most toxic dye and TA (8 mg/ml without preservative) showed least toxic to cells. The toxicty of dyes was dose and time-dependent.

Conclusions:: In this study, TA (8 mg/ml without preservative) is less toxic in cell culture when compare to the other clinical staining materials.

Keywords: retinal pigment epithelium • retinal glia • apoptosis/cell death 
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