May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
A New Method to Detect Retinal Vascular Leakage With HRA2
M. Fujihara; K. Yamashiro; Y. Kurimoto; M. Takahashi; T. Asahara; J. T. Handa
Author Affiliations & Notes
  • M. Fujihara
    Ophthalmology-Retinal Vascular Service, Johns Hopkins University School of Medicine, Baltimore, Maryland
  • K. Yamashiro
    Department of Opthalmology, Kobe City General Hospital, Kobe, Japan
    Department of Regenerative Medicine, Institute of Biomedical Research and Innovation, Kobe, Japan
  • Y. Kurimoto
    Department of Opthalmology, Kobe City General Hospital, Kobe, Japan
    Department of Regenerative Medicine, Institute of Biomedical Research and Innovation, Kobe, Japan
  • M. Takahashi
    Department of Opthalmology, Kobe City General Hospital, Kobe, Japan
    Center for Developmental Biology, Riken Kobe Institute, Kobe, Japan
  • T. Asahara
    Department of Regenerative Medicine, Institute of Biomedical Research and Innovation, Kobe, Japan
    Center for Developmental Biology, Riken Kobe Institute, Kobe, Japan
  • J. T. Handa
    Ophthalmology-Retinal Vascular Service, Johns Hopkins University School of Medicine, Baltimore, Maryland
  • Footnotes
    Commercial Relationships M. Fujihara, None; K. Yamashiro, None; Y. Kurimoto, None; M. Takahashi, None; T. Asahara, None; J.T. Handa, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 2579. doi:
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      M. Fujihara, K. Yamashiro, Y. Kurimoto, M. Takahashi, T. Asahara, J. T. Handa; A New Method to Detect Retinal Vascular Leakage With HRA2. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2579.

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Abstract

Purpose:: Retinal edema can result in severe visual loss. An easy method to detect retinal vascular leakage in an animal model of retinal edema would be indispensable for investigation of new treatment strategy. The purpose of the current study was to establish a new method for detecting retinal vascular leakage in endotoxin-induced uveitis (EIU) in a mouse with HRA2.

Methods:: EIU was induced in C57BL/6J mice (n=51) by intravitreous injection of lipopolysaccharide (LPS). At various time points (0, 24, 48, 72hours) after LPS injection, fluorescein sodium salt solution was injected intraperitoneally. The fundus was observed with HRA2 using fluorescein angiography 24 hours after fluorescein injection.

Results:: The retinal vascular leakage could be detected as remnant fluorescence of the retina in all EIU mice and could not be detected in normal. The degree of the leakage could be easily classified from stage 0 to stage 4 and these correlate with the injected volume of LPS.

Conclusions:: We successfully established a novel method to detect retinal vascular leakage in EIU mice. Our new method would be a great help to develop new treatment strategies for retinal edema.

Keywords: imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • uveitis-clinical/animal model • inflammation 
© 2007, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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