May 2007
Volume 48, Issue 13
ARVO Annual Meeting Abstract  |   May 2007
Role of Immune Inhibitors InhA and InhA2 in Experimental Bacillus Endophthalmitis
Author Affiliations & Notes
  • J. J. Hunt
    Microbiology and Immunology, Univ of Oklahoma HSC, Oklahoma City, Oklahoma
  • B. D. Novosad
    Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma
  • M. C. Callegan
    Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma
    Molecular Pathogenesis of Eye Infections Research Center, Dean A. McGee Eye Institute, Oklahoma City, Oklahoma
  • Footnotes
    Commercial Relationships J.J. Hunt, None; B.D. Novosad, None; M.C. Callegan, None.
  • Footnotes
    Support NIH grant R01EY12985 and Lew R. Wasserman Award from Research to Prevent Blindness, Inc. (to MCC) and an NIH CORE grant P30 EY12191 (R.E. Anderson)
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 2664. doi:
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      J. J. Hunt, B. D. Novosad, M. C. Callegan; Role of Immune Inhibitors InhA and InhA2 in Experimental Bacillus Endophthalmitis. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2664.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose:: To ascertain the role of bacterial immune inhibitors InhA and InhA2 in experimental Bacillus endophthalmitis.

Methods:: Bacillus thuringiensis strain 407 (WT) and isogenic mutants of immune inhibitors A and A2 (InhA, InhA2) were tested in the rabbit model of post-traumatic experimental endophthalmitis. Eyes were injected intravitreally with 100 CFU of strain BT407, InhA-, InhA2-, or InhA-/InhA2-. Pathogenesis was monitored by electroretinography, bacterial enumeration, and histology.

Results:: The intraocular growth rates of Bacillus mutants InhA2- and InhA-/InhA2- were significantly higher than that of WT and InhA- at early time points, but all strains reached similar intraocular numbers by 12 hours postinfection. Interestingly, all Inh mutants caused near complete loss of retinal function by 12 hours postinfection, while animals infected with the wild type strain retained 40% B-wave function by electroretinogram. Additionally, Inh mutants exhibited increased kinetics of ERG B-wave loss during the course of infection. Intraocular inflammation reached severe levels by 12 hours postinfection in eyes infected with each strain.

Conclusions:: Both wild type Bacillus and its Inh mutants had a devastating effect, causing severe visual function loss and significant inflammation by 12 hours postinfection. However, the presence of InhA and InhA2 may have been responsible for a slight attenuation of infection. Overall, the results suggested that InhA and InhA2 may not play a prominent role in the pathogenesis of experimental Bacillus endophthalmitis.

Keywords: bacterial disease • endophthalmitis • microbial pathogenesis: experimental studies 

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