May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Topical Iv-IgG Inhibits Adenovirus Replication in the Ad5/NZW Rabbit Ocular Model
Author Affiliations & Notes
  • E. G. Romanowski
    The Charles T. Campbell Laboratory, UPMC Eye Center, Ophthalmology and Visual Sciences Research Center, Eye and Ear Institute, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania
  • E. Nwanegbo
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania
  • K. A. Yates
    The Charles T. Campbell Laboratory, UPMC Eye Center, Ophthalmology and Visual Sciences Research Center, Eye and Ear Institute, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania
  • A. Gambotto
    Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania
  • Y. J. Gordon
    The Charles T. Campbell Laboratory, UPMC Eye Center, Ophthalmology and Visual Sciences Research Center, Eye and Ear Institute, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania
  • Footnotes
    Commercial Relationships E.G. Romanowski, None; E. Nwanegbo, None; K.A. Yates, None; A. Gambotto, Inventor, P; Y.J. Gordon, None.
  • Footnotes
    Support NIH Grant EY05323 (YJG), NIH Core Grant EY08098 (OVSRC), University of Pittsburgh Office of Technology Management Grant 01220, Eye & Ear Foundation of Pittsburgh, RPB.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 2670. doi:
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      E. G. Romanowski, E. Nwanegbo, K. A. Yates, A. Gambotto, Y. J. Gordon; Topical Iv-IgG Inhibits Adenovirus Replication in the Ad5/NZW Rabbit Ocular Model. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2670.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

Presently, there is no FDA approved antiviral therapy for the treatment of adenovirus (Ad) ocular infections. The goal of the current study was to determine the antiviral efficacy of Intravenous Immunoglobulin G (Iv-IgG), a preparation of highly purified and concentrated immunoglobulin G (IgG) antibodies isolated from a large pool of human plasma donors, on acute Ad replication in the Ad5/NZW rabbit ocular model.

 
Methods:
 

15 NZW rabbits were topically inoculated in both eyes, following corneal scarification, with 1.5 x 106 pfu/eye of Ad5. On day 1, the rabbits were divided into 3 topical treatment groups (n=5/group): I - 10% Iv-IgG, QID x 10 days; II - 0.5% Cidofovir (CDV), BID x 7 days; III - Control (saline), QID x 10 days. All eyes were cultured for virus on days 0, 1, 3, 4, 5, 7, 9, 11, and 14.

 
Results:
 

* p < 0.02 compared to the Control. p = 0.003 compared to CDV.  

 
Conclusions:
 

Topical 10% Iv-IgG was significantly more effective than the Control in reducing Ad Positive Cultures/Total (Days 7-14), Duration of Ad Shedding, Mean Ad Titer (Days 1-5), and Mean Ad Titer (Days 7-14) in the Ad5/NZW rabbit ocular model. Furthermore, 10% Iv-IgG was more effective than 0.5% Cidofovir in some viral outcome measures. Additional studies are warranted to establish the clinical potential of Iv-IgG as a topical antiviral treatment for adenovirus ocular infections.

 
Keywords: adenovirus • antiviral drugs • conjunctivitis 
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