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T. Sumioka, S. Saika, K. Ikeda, Y. Okada, O. Yamanaka, Y. Ohnishi; Adenoviral Gene Transfer of Smad7 Suppresses Epithelial-Mesenchymal Transition and Fibrogenic Reaction by Corneal Endothelium Post-Alkali-Burn in Rats. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2698.
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To examine if overexpression of Smad7 by adenoviral gene transfer suppresses epithelial-mesenchymal transition and subsequent fibrogenic reaction by corneal endothelium in an alkali-burned cornea in rats. Such reaction causes formation of retrocorneal membrane in an alkali-burned cornea.
Two adenoviral vectors carrying Cre recombinase under CAG promoter and Smad7 cDNA under LNL promoter, mixed with hyaluronan, were applied to the anterior chamber of adult Wistar rats (n = 14) under general anesthesia. Control animals received a vector of CAG/Cre recombinase. After 3 days the eye received 10 microL of 1N NaOH. After specific interval of healing the animals were killed and the eye was processed for histology/immunohistochemistry for α-smooth muscle actin (αSMA) and collagen I. Efficacy of gene transfer was evaluated by the vector carrying green fluorescein protein (GFP).
GFP was detected in corneal endothelium. In control healing cornea αSMA was expressed in stromal fibroblasts and corneal endothelium. Collagen I was also seen in endothelium. Expression of αSMA and collagen I was suppressed in corneal endothelium.
Smad7 overexpression suppresses epithelial-mesenchymal transition and subsequent fibrogenic reaction by corneal endothelium in an alkali-burned cornea in rats.
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