May 2007
Volume 48, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2007
Protective Effects of Ovophospholipids Against Oxidative Stress and Senescence
Author Affiliations & Notes
  • Y. Ryu
    Department of Ophthalmology, Chung-Ang University Medical Center, Seoul, Republic of Korea
  • J. Rhim
    Department of Biochemistry, College of Medicine, Seoul National University, Seoul, Republic of Korea
  • H. Lee
    Department of Ophthalmology, Chung-Ang University Medical Center, Seoul, Republic of Korea
  • S. Park
    Department of Biochemistry, College of Medicine, Seoul National University, Seoul, Republic of Korea
  • J. Kim
    Department of Ophthalmology, Chung-Ang University Medical Center, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships Y. Ryu, None; J. Rhim, None; H. Lee, None; S. Park, None; J. Kim, None.
  • Footnotes
    Support None.
Investigative Ophthalmology & Visual Science May 2007, Vol.48, 2719. doi:
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    • Get Citation

      Y. Ryu, J. Rhim, H. Lee, S. Park, J. Kim; Protective Effects of Ovophospholipids Against Oxidative Stress and Senescence. Invest. Ophthalmol. Vis. Sci. 2007;48(13):2719.

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Abstract

Purpose:: To identify the possibility of senescence retardation in human corneal cells by investigating the cellular protective effects of ovophospholipids (OPL) against oxidative stress.

Methods:: The proliferation and protective effects of OPL was assessed by MTT assay in 0.5-3.0% OPL-treated human corneal epithelial (HCEC) and stromal (HCSC) cells. Cell cycle analysis was performed by RT-PCR of cyclins and CDKs. Messenger RNA expressions of IL-1 and MMP-9 were also investigated by RT-PCR. The expression of senescence marker, ß-galactosidase, was compared between high and low passage numbers of HCEC and HCSC by X-gal staining.

Results:: OPL-treated HCEC and HCSC showed retarded cell proliferation, but they did not showed any evidence of cell death. The mRNA expression of IL-1ß was decreased in OPL-treated human corneal cells, dose dependently. The ß-galactosidase expression levels between control and OPL-treated different passage numbers of cells did not significantly different.

Conclusions:: OPL treatment can protect the human corneal cells against oxidative stress, but more studies are required to identify their senescence retardation activities.

Keywords: aging • cornea: basic science • oxidation/oxidative or free radical damage 
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